On 10/02/11 16:56, Jacob Keller wrote:
Dear Crystallographers,
I would like to soak my crystals in bicarbonate (a possible
substrate), but the crystals have grown--and only grow--in pH 5.2-6.0,
so the bicarb/CO2 will just keep evolving out of the solution and
reliquishing its hydroxyls until
Hi Jacob, high-pressure cryocrystallography methods may be useful in this case.
I copied your question to Chae Un Kim here at MacCHESS and he forwards this
suggestion:
--
Hi Richard,
I think pressure cryocooling might be useful.
They may want to check
Further Qs.
Do you have a noncryst translation parallel to the b axis (ctruncate
will list any such translation..)
If the b shift is 0.5 then the 0k0 absences will be present whether
the spacegroup is P2 or P21.
How many Xe sites do you expect? If there is only one then phasing is
more
Why not just go to P1, then build up the symmetry? Is completeness low?
JPK
On Mon, Oct 3, 2011 at 10:14 AM, Eleanor Dodson c...@ysbl.york.ac.uk wrote:
Further Qs.
Do you have a noncryst translation parallel to the b axis (ctruncate will
list any such translation..)
If the b shift is 0.5
On Sun, 2011-10-02 at 15:56 -0500, Jacob Keller wrote:
Transfer them to higher pH, and hope
for the best?
Consider crosslinking your crystals with glutaraldehyde. They will then
became virtually insoluble, although it's possible that you may lose
diffraction at elevated pH.
--
Hurry up
Hi Jacob,
Once I had an issue with introuding CO2 into crystals and I was able to get it
bound by putting a small piece of dry ice into the well solution and keeping
the drop under CO2 pressure using nextal trays with screw cap. Please note in
this case CO2 didn't get bound by using pressure
Hi,
I am trying to calculate real-space R-factors and correlation coefficients for
an array of different ligand conformations to find out which fits best in
experimental density. So far, I have been trying to use Overlapmap in CCP4
6.1.2 to do this, by correlating maps by residue and
Sorry for the very off topic and dumb question, but does anyone know if BeCl2
needs to be prepared fresh for use (making BeF3) or can it be stored as a
solution stock at room temperature/frozen?
Thanks,
Peter
Hi Peter,
Both BeF3(-) and AlF4(-) need to be made fresh, or used from frozen. It
should not be stored at room temperature. While using, you may wish to keep
it on ice. If you are using Vanadate, that should be fresh (Methods in
Enzymology).
Also kindly note that unlike AlF4(-), which is a
