Dear ALL;
Recently, one of my colleagues cloned a gene (200aa) into pET30a vectors
with either a N-ter or C-ter His6 tag. The correct reading frame was confirmed
by sequencing.
However, it is weird that there was no protein expression either in the
soluble fraction or as
pseudo translation is very common and usually does not pose a serious
problem. you have to be careful about assigning the space group, since a
translation od x,y,z=1/2 will generate absences for l = 2n+1 which may
suggest a screw axis along c . But since your space group is P21 that isn't
a
Hi Jerry:
There are many instances where we miss going through process of sequencing
the entire reading frame, as many sequencing cores provides sequences only
upto 600-700 bases, I would recommend sequencing a complete reading frame
using mid frame primers and then conclude whether the reading
Hello,
Sorry for the question if non-ccp4. I am using a HP workstation Xeon Z400.
This has both windows and linux Mint. I am using crystallographic programs
with stereo setup in coot, o, pymol, vmd…. I bought a new exactly same
desktop with all same hardware configurations except the old one has
On Sat, 2012-03-17 at 08:44 -0400, Hena Dutta wrote:
I tried with clonezill and it did not work.
There are many problems with what you are trying to do.
1. Windows license is tied to the hardware, thus it's not likely to
work out of the box if you clone the whole drive.
2. Even though it's
One comment I'd like to add here is that in the presence of
pseudo-translational ncs that is nearly colinear with crystal axes you
will have a significantly higher R-value. This may be a serious problem
with some reviewers when your R~30% on a 2A dataset. It is completely
justified then to have
