[ccp4bb] 24/25 May 2012 IGBMC symposium on “Future Challenges in Integrative Structural Biology”

2012-03-30 Thread Bruno KLAHOLZ
Dear all, kind reminder for the Instruct/FRISBI symposium at the IGBMC on 24/25 of May on “Future Challenges in Integrative Structural Biology” since the deadline (april 9th 2012) is approaching. Seehttp://fcisb2012.u-strasbg.fr for registration. The conference includes

[ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread Kevin Jin
Dear All, Here is way I have used for protein and hydrophobic organic compound cocrystallization. Via this method, less amount of DMSO will be used to aviod protein ppt. I hope you can use for your research. http://www.jinkai.org/Xtal.html Regards, -- Kevin Jin Sharing knowledge each other

Re: [ccp4bb] an ambiguous result of molecular replacement

2012-03-30 Thread Randy Read
Hi, Do you mean that the second molecule is always overlapped with the first, by saying that it shifts several Angstrom along the x axis? If there were a larger translation, then what you're seeing would be consistent with translational NCS (tNCS), but the translation should be large enough

Re: [ccp4bb] processing data with ice ring

2012-03-30 Thread Harry Powell
Hi Actually, it's *much* easier than this in iMosflm (though Sita's suggestion is already pretty easy). There's no need to open the Processing Options menu in this case. On each of the indexing, the refinement and integration panes, there is a small button on the top row of widgets with

Re: [ccp4bb] processing data with ice ring

2012-03-30 Thread Loes Kroon-Batenburg
Dear Shanti, You may want to try EVAL (www.crystal.chem.uu.nl/distr/eval). It provides options to exclude regions affected with ice diffraction, or practically any region you'd like. In our experience data treated in this way have a lower Rmerge. However, the difference with respect to

[ccp4bb] about heavy atom derivatization

2012-03-30 Thread Shanti Pal Gangwar
Dear all I am beginner in crystallography.We have collected a native data of a given protein at 2.2A resolution but are unable t solve by MR therefore we collected Hg, and Pt derivative after soaking the crystals. I don't know how to process this heavy atom derivative data and find the

Re: [ccp4bb] about heavy atom derivatization

2012-03-30 Thread Ed Pozharski
http://www.ccp4.ac.uk/dist/examples/tutorial/html/heavy-tutorial-mir.html seems relevant On Fri, 2012-03-30 at 19:04 +0530, Shanti Pal Gangwar wrote: Dear all I am beginner in crystallography.We have collected a native data of a given protein at 2.2A resolution but are unable t solve by MR

Re: [ccp4bb] about heavy atom derivatization

2012-03-30 Thread Francis E Reyes
To add to Ed's comments: Find someone in the immediate department/area to walk you through your first structure. But to answer your question: What about the MR didn't work? Use your MR model to find your heavy atoms. They can be found using anomalous data difference fouriers (or even

[ccp4bb] Announcement: EMBO Global Exchange Lecture Course: Structural and biophysical methods for biological macromolecules in solution

2012-03-30 Thread Clement Blanchet
Structural and biophysical methods for biological macromolecules in solution EMBO Global Exchange Lecture Course 29 November?6 December 2012 Hyderabad, India http://events.embo.org/12-macromolecule/index.html The main objective of the Course is to teach the young PhD students and postdocs

Re: [ccp4bb] need help for crystallization

2012-03-30 Thread Kevin Jin
My pleasure ! Since this may be help for other people, I also cc it to CCP4BBS. According to your email, I guess the buffer is phosphate buffer at pH 7.4. You can do a quick buffer exchange before crystallization. Since PO4 is a competitor in this case, I will also avoid PO4 and Cocodylate

Re: [ccp4bb] an ambiguous result of molecular replacement

2012-03-30 Thread Leonid Sazanov
Hi, we had the same case in apparent C2221, with many similarly shifted Phaser solutions with high scores. The reason was that crystals were actually nearly perfectly twinned in P21, so indexing and processing indicated C2221. Once data was re-processed in P21, Phaser could easily find two

Re: [ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread Bryan Lepore
On Fri, Mar 30, 2012 at 4:02 AM, Kevin Jin kevin...@gmail.com wrote: Here is way I have used for [...] I hate to be a curmudgeon, but can a list member please explain why this is not specifically blogspam or spam - or whatever it is exactly? -Bryan

Re: [ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread Jacob Keller
What's the harm? Seems relevant to crystallographers, and not for self-promotion, but just to help, share an interesting tip. Perhaps you can think of it as a response to an un-asked but plausible question, i.e., how can I treat my coverslips to make them more receptive to organic

Re: [ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread Bryan Lepore
On Fri, Mar 30, 2012 at 3:26 PM, Jacob Keller j-kell...@fsm.northwestern.edu wrote: What's the harm? have you seen usenet/Google groups? -Bryan

Re: [ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread James Stroud
On Mar 30, 2012, at 1:04 PM, Bryan Lepore wrote: On Fri, Mar 30, 2012 at 4:02 AM, Kevin Jin kevin...@gmail.com wrote: Here is way I have used for [...] I hate to be a curmudgeon, but can a list member please explain why this is not specifically blogspam or spam - or whatever it is exactly?

[ccp4bb] Reminder - Gordon Research Conference on Diffraction Methods

2012-03-30 Thread Ana Gonzalez
We would like to remind you of the biannual Gordon Conference on Diffraction Methods in Structural Biology, which will be held at Bates College in Lewiston, Maine from July 15-20. We are closer to the application deadline now (June 17th), and places will be filling up pretty fast! We have

Re: [ccp4bb] a small trick for protein and organic compound cocrystallization.

2012-03-30 Thread Zhijie Li
Hi, If those are spams, then Kevin must be the most successful spammer I have ever encountered, as I have actually read all his posts and linked web pages. About the twitter idea, I am not sure if I would ever become a twitter user in the near future. Compared to following tens of authors,