Dear all,
kind reminder for the Instruct/FRISBI symposium at the IGBMC on 24/25 of May on
“Future Challenges in Integrative Structural Biology” since the deadline (april
9th 2012) is approaching. Seehttp://fcisb2012.u-strasbg.fr for
registration.
The conference includes
Dear All,
Here is way I have used for protein and hydrophobic organic compound
cocrystallization. Via this method, less amount of DMSO will be used
to aviod protein ppt.
I hope you can use for your research.
http://www.jinkai.org/Xtal.html
Regards,
--
Kevin Jin
Sharing knowledge each other
Hi,
Do you mean that the second molecule is always overlapped with the first, by
saying that it shifts several Angstrom along the x axis? If there were a
larger translation, then what you're seeing would be consistent with
translational NCS (tNCS), but the translation should be large enough
Hi
Actually, it's *much* easier than this in iMosflm (though Sita's
suggestion is already pretty easy). There's no need to open the
Processing Options menu in this case.
On each of the indexing, the refinement and integration panes, there
is a small button on the top row of widgets with
Dear Shanti,
You may want to try EVAL (www.crystal.chem.uu.nl/distr/eval). It provides
options to exclude regions affected with ice diffraction, or practically any
region you'd like. In our experience data treated in this way have a lower
Rmerge. However, the difference with respect to
Dear all
I am beginner in crystallography.We have collected a native data of a given
protein at 2.2A resolution but are unable t solve by MR
therefore we collected Hg, and Pt derivative after soaking the crystals.
I don't know how to process this heavy atom derivative data
and find the
http://www.ccp4.ac.uk/dist/examples/tutorial/html/heavy-tutorial-mir.html
seems relevant
On Fri, 2012-03-30 at 19:04 +0530, Shanti Pal Gangwar wrote:
Dear all
I am beginner in crystallography.We have collected a native data of a
given protein at 2.2A resolution but are unable t solve by MR
To add to Ed's comments:
Find someone in the immediate department/area to walk you through your first
structure.
But to answer your question:
What about the MR didn't work?
Use your MR model to find your heavy atoms. They can be found using anomalous
data difference fouriers (or even
Structural and biophysical methods for biological macromolecules in solution
EMBO Global Exchange Lecture Course
29 November?6 December 2012 Hyderabad, India
http://events.embo.org/12-macromolecule/index.html
The main objective of the Course is to teach the young PhD students
and postdocs
My pleasure !
Since this may be help for other people, I also cc it to CCP4BBS.
According to your email, I guess the buffer is phosphate buffer at pH
7.4. You can do a quick buffer exchange before crystallization.
Since PO4 is a competitor in this case, I will also avoid PO4 and
Cocodylate
Hi, we had the same case in apparent C2221, with many similarly shifted Phaser
solutions with high scores. The reason was that crystals were actually nearly
perfectly twinned in P21, so indexing and processing indicated C2221. Once data
was re-processed in P21, Phaser could easily find two
On Fri, Mar 30, 2012 at 4:02 AM, Kevin Jin kevin...@gmail.com wrote:
Here is way I have used for [...]
I hate to be a curmudgeon, but can a list member please explain why
this is not specifically blogspam or spam - or whatever it is exactly?
-Bryan
What's the harm? Seems relevant to crystallographers, and not for
self-promotion, but just to help, share an interesting tip. Perhaps you can
think of it as a response to an un-asked but plausible question, i.e., how
can I treat my coverslips to make them more receptive to organic
On Fri, Mar 30, 2012 at 3:26 PM, Jacob Keller
j-kell...@fsm.northwestern.edu wrote:
What's the harm?
have you seen usenet/Google groups?
-Bryan
On Mar 30, 2012, at 1:04 PM, Bryan Lepore wrote:
On Fri, Mar 30, 2012 at 4:02 AM, Kevin Jin kevin...@gmail.com wrote:
Here is way I have used for [...]
I hate to be a curmudgeon, but can a list member please explain why
this is not specifically blogspam or spam - or whatever it is exactly?
We would like to remind you of the biannual Gordon Conference on Diffraction
Methods in Structural Biology, which will be held at Bates College in
Lewiston, Maine from July 15-20. We are closer to the application
deadline now (June 17th), and places will be filling up pretty fast!
We have
Hi,
If those are spams, then Kevin must be the most successful spammer I have
ever encountered, as I have actually read all his posts and linked web
pages.
About the twitter idea, I am not sure if I would ever become a twitter user
in the near future. Compared to following tens of authors,
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