[ccp4bb] Indexing problem

Dear All, A protein with SG P212121 co-crystallized with Benzamidine, cell dimension a=52.8, b=54.6, c= 82.90(reported). when I am going to reproduce the crystal with reported condition I am getting crystals. I am trying to index the frame (DENZO), it is showing P222 /P1 space group but the

Re: [ccp4bb] Indexing problem

Dear Dipankar, it happens quite frequently that using the same conditions, on still gets a different crystal form. Just process the data with the space group proposed by DENZO and run a molecular replacement program (e.g. Phaser) to solve the structure. You need to switch on the SGALTERNATIVE ALL

[ccp4bb] Post-doc position - Umea University, Sweden

A two year post-doctoral fellowship is available in the group of Elisabeth Sauer-Eriksson and Tobias Hainzl at Umea University, Sweden. The project will involve structural characterizations of RNA and RNA-protein complexes based on X-ray crystallographic methods. Ideally, the applicant

[ccp4bb] Layer Groups: B2 recognized?

Hello Everyone and especially symmetry experts, I found at http://img.chem.ucl.ac.uk/sgp/medium/003dy1.htm that B2 (SG #2) is a recognized space group that has a basis rotated from P2 (and as a result has two new positions). I'm wondering whether a similar B2 for P2 (LG #3) is recognized for

[ccp4bb] NCS on multiple conformations

Dear all, ok, never mind. I defined either peptide conformation as part of the protein it binds to and applied NCS to that. Should have thought of that before sending out the question. Andreas -- Andreas Förster, Research Associate Paul Freemont Xiaodong Zhang Labs

[ccp4bb] Postdoc position

One postdoctoral position funded by NIH is available immediately at the Center for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School (http://www.uth.tmc.edu/cmb/). The fellow will focus on structural determination of important

[ccp4bb] NCS on multiple conformations

Dear all, I have solved the structure of a protein with twofold NCS. On the axis relating the protein molecules sits a peptide ligand in two (mutually exclusive, partially occupied) conformations, effectively interacting with either protein molecule half of the time. Sorting out the side

[ccp4bb] PhD position available at Umea University, Sweden

Dear All, A full time PhD student position is available in the laboratory of Uwe Sauer at the Chemical Biology Center at Umea University, Sweden. The project focuses on proteins involved in oxygenic photosynthesis. The successful candidate will determine 3D structures by X-ray Crystallography

[ccp4bb] Off-topic: 96-well plate PCR/plasmid purification

Dear All, I am looking for a bit of advice, and am interested to hear about experiences people have had with various commercially-available 96- well plate-based PCR, plasmid, and protein purification appliances and plates. Forgive me for comparing commercial vendors. We have a 96-well

[ccp4bb] Protein thermostability

Dear all I have two homologues structures, from a mesophilic bacterium and a thermophilic bacterium. Is there any software or server that can calculate the differences that contribute to thermostability, e.g. proportion of amino acids that form Hydrogen bonds and number of prolines? Thank

Re: [ccp4bb] Protein thermostability

Hi Hsu, You can check my paper. We have done a similar analysis of several factors responsible for thermal stability, analysis done using various mesophiles and thermophiles, Crystal structure of the *Bacillus anthracis* nucleoside diphosphate kinase and its characterization reveals an enzyme