On 7/13/2012 1:58 AM, Tim Gruene wrote:
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Dear all,
I am surprised by the discussion about chiraliy of an utterly
centrosymmetric molecule. Shouldn't the four Oxygen atoms be at least
from a QM point-of-view to indistinguishable? What reason is there
Dear All,
I am interested in cross-linking of a protein molecule with a complex
(isolated). The protein is expressed recombinantly from E.coli and we are
would like to precisely map the residues involved in interaction. So far,
all other crosslinking methods (targeting amines etc.) have not
On Sunday, 15 July 2012, Dale Tronrud wrote:
There are good reasons for maintaining order in this human-induced
numbering scheme. A common operation is to superimpose two molecules
and calculate the rmsd of the positional differences. This calculation
is not useful when the Val CG1 and
On Jul 13, 2012, at 4:00 PM, Christian Roth wrote:
I want align a couple or protein structures by secondary structure matching
to
one target and want get a kind of aminoacid alignment file e.g. what residue
fit
the other, without adjustments due to sequence based alignments.
I tried
Dear all,
Sorry for non-crystallography related question.
We are trying to express an eukaryotic heme protein in E. coli. We are able to
express it in soluble form. When we use 5-Aminolevulinic Acid, E. coli becomes
brown. However, after cell lysis the soluble protein contains no heme and the
