[ccp4bb] Technician position available at the SLS

2013-06-04 Thread May Marsh
http://www.psi.ch/pa/offenestellen/0595-1 We are looking for a Technician Protein Crystallization Facility and Crystallography Beamlines Your tasks You will be responsible for helping with the everyday operation and maintenance of the crystallization and liquid handling robots within the

[ccp4bb] visualizing protein-protein interface

2013-06-04 Thread Tobias Beck
Dear all, I would like to visualize the interface of a protein-protein complex. The complex formation is modulated by a ligand that upon binding alters the overall structure of one protein, which then in turn forms the complex with the second protein (structure of the complex is known). Ligand

Re: [ccp4bb] visualizing protein-protein interface

2013-06-04 Thread Bosch, Juergen
Hi Tobias, this may or may not work. calculate a map for both proteins, normalize them, put them on an identical cell and subtract one map from another. The program that can do this is called mapman *gosh* a non-CCP4 thing. Good luck, Jürgen On Jun 4, 2013, at 10:18 AM, Tobias Beck wrote:

[ccp4bb] protein tags

2013-06-04 Thread Anindito Sen
Dear All, We are looking to protein tags (of size not exceeding 10 kDa) as markers to label our protein complex of size ~50 kDa. Although I have searched for several possible candidates mainly mini-proteins, most of them are not what we want to have. The features that we are looking in the tag

[ccp4bb] Helix forming proteins crystallizing as rings

2013-06-04 Thread Joern Krausze
Dear all, can anyone provide me with examples for proteins that form a helical oligomer under physiological conditions and flat rings (basically a helix with a pitch of 0) in the crystal? I only know examples of the other way around. Thank you in advance. Joern

[ccp4bb] Postdoctoral position at University of Leipzig, Germany

2013-06-04 Thread Norbert Straeter
A full-time postdoctoral position is available at the Institute of Bioanalytical Chemistry, University of Leipzig, Germany. We are seeking an independent and highly motivated postdoctoral researcher for protein design and structural studies on redox enzymes in biocatalytic applications,

Re: [ccp4bb] visualizing protein-protein interface

2013-06-04 Thread eugene . krissinel
This is the kind of things MG was designed to do -- Eugene On 4 Jun 2013, at 15:18, Tobias Beck wrote: Dear all, I would like to visualize the interface of a protein-protein complex. The complex formation is modulated by a ligand that upon binding alters the overall structure of one protein,

[ccp4bb] Re : Re: [ccp4bb] visualizing protein-protein interface

2013-06-04 Thread Nadir Mrabet
Google 2D-GraLab. Very good at showing detailed interface contacts along protein sequences. To my knowledge, there is no way to include a non protein ligand. But the source code is available on request and if you succeed to modify the program, please let us know. HTH, Nadir Mrabet Pr.

[ccp4bb] Glutathione rsin

2013-06-04 Thread Cygler, Miroslaw
Hi, I would like to ask the bb faithful for their experience with the glutathione affinity resins. We have been using so far the Glutathione Sepharose fast flow from GE but the price is getting steeper. We found Glutathione Superflow resin from Clontech to be significantly less expensive and