[ccp4bb] Protein concentration for crystallization

What would be a convenient way to estimate what percentages of proteins have been crystallized in a concentration range, for example 5-30 mg? Debasish Chattopadhyay University of Alabama at Birmingham CBSE-250 1025 18th Street South, Birmingham, Al-35294 USA Ph: (205)934-0124; Fax:

Re: [ccp4bb] Protein concentration for crystallization

Dear Debasish, you can use REMARK 200 field in pdb file. Sadly, this field is not mandatory so not everyone provide protein concentration info. 10.06.2013 18:49, Debasish Chattopadhyay ?: What would be a convenient way to estimate what percentages of proteins have been crystallized in a

[ccp4bb] Hi

Hi all, I was trying to solve the structure of a protein in several different datasets using xds and phenix. I could solve the structure from one dataset in space group P4. For another dataset, I could solve the structure using the monomer of the structure I got from the first dataset as search

Re: [ccp4bb] Protein concentration for crystallization

Dear Debasish What do you mean by percentage? do you mean consentration? so if you mean cons. I think you should test you protein using a TCP kit to observe at what cons. would your protein  precipitate, this way you would verify the convinient cons.  for your protein before crystallization

Re: [ccp4bb] Hi

I don't really understand what your space group is? space group mC??? Eleanor On 10 Jun 2013, at 19:57, Wei Shi wrote: Hi all, I was trying to solve the structure of a protein in several different datasets using xds and phenix. I could solve the structure from one dataset in space group

Re: [ccp4bb] Hi

Hi Eleanor, C2 - this was XDS lingo or Bravais talk :-) Jürgen ** LATTICE SYMMETRY IMPLICATED BY SPACE GROUP SYMMETRY ** BRAVAIS-POSSIBLE SPACE-GROUPS FOR PROTEIN CRYSTALS TYPE [SPACE GROUP NUMBER,SYMBOL] aP [1,P1] mP [3,P2] [4,P2(1)] mC,mI

Re: [ccp4bb] Hi

The other obvious conclusion would be that dataset #3 is a different protein perhaps ? How about pointless for your third dataset ? Jürgen On Jun 10, 2013, at 2:57 PM, Wei Shi wrote: Hi all, I was trying to solve the structure of a protein in several different datasets using xds and phenix. I

Re: [ccp4bb] Hi

I guess Wei means just the lattice symbol, taken from the indexing program? br -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Eleanor Dodson Sent: Monday, June 10, 2013 10:52 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Hi I don't really

Re: [ccp4bb] Off-topic: NMR and crystallography

Hi Theresa, I think you have to be very careful with NMR of homo-oligomers, even if they’re small proteins: the NMR model/structure (backbone only) of a small integral membrane kinase was a huge effort - http://www.ncbi.nlm.nih.gov/pubmed/19556511 but is very different from the recently

Re: [ccp4bb] Protein concentration for crystallization

Perhaps my question was not expressed well. I wanted to know if proteins crystallize more frequently when the protein concentration is in the range 5-30mg/ml. The answer pointed out by my colleague Todd Green is on the page http://www.douglas.co.uk/PDB_data.htm Thanks for your inputs.