Peter Hsu wrote:
Hi all,
I've generally always thought as long as the peak was symmetrical and not too
broad would suggest a good sample. However, looking at my previous runs in the
past, I've had peaks as narrow as 1.5-2mL on a 24mL SD200, or slightly broader
peaks with about 3mL (all
Hi Ed,
I guess by 24mL SD200 Peter meant the Superdex 200 10/300 column, which
most of us should be quite familiar with. According to GE healthcare, a new
Superdex 200 10/300 GL column should have TP 25000/m. For comparison, a new
Superdex200 16/600 PG, which uses bigger beads, has TP
Hi Peter,
I won't claim to be an expert, however, there is a small textbook by Robert K.
Scopes called Protein Purification: Principles and Practice, that has very
good practical and theoretical information about column chromatography (among
other methods). I have found this book useful a
Thanks- guess I'm old-fashioned, using low-pressure columns.
So apparently theoretical plates are still calculated, and have
improved a lot- 25000/m is HETP .04 mm, way better than the
figure I mentioned. (TP per dollar not so much.)
No more sour grapes from me-
eab
Zhijie Li wrote:
Hi Ed,
I
