Jacob,
Background subtraction is unfortunately not as forgiving as you think it
is. You can subtract the background, but you can't subtract the noise.
This is because noise (by definition) is never the same twice. Yes, the
average or true background under a spot may be flat, but any given
Dear Jacob,
To add one more thing to the many ones from James, despite air scattering noise
being flat and easily subtractable over time, that noise wont be the same if
you take images with and without the sample. Since some of the photons will be
absorbed and diffracted by the crystal, the
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dom
Bellini
Sent: Thursday, January 15, 2015 12:25 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does
rad dam travel?)
Dear Jacob,
To add
Dear CCP4ers,
We are looking for a highly motivated PostDoc that defended the thesis
between January 1st 2010 and December 31st 2012 to apply for a Juan de la
Cierva - *Incorporación *2-years contract at the Spanish Ministry of
Economy and Competitiveness (BOE 5 Diciembre 2014, Sec III, pág
If your density is good enough (i.e. some SS elements at least are clear)
and the resolution permits (generally 3A or better, but sometimes even
3.3-3.5A) then my favorite strategy is to autobuild parts of the model,
hand-validate the builds (i.e. prune the autobuit model such that only high
Lawrence Berkeley Laboratory
Career-track Research Scientist
Job ID: 80447
Division: Physical Biosciences
Date Opened: 1/14/2015
https://lbl.taleo.net/careersection/2/jobdetail.ftl?lang=enjob=80447
Summary: A research scientist position is available to work on developing new
methods for
I think a summary is that:
Background levels and errors thereof can be estimated very precisely as a
percentage of the level, but as an absolute number, it nevertheless swamps out
the signal contained in a Bragg peak. For example, if background = 10^6 photons
+/- 1000 (very good estimate as a
Dear All
We would like to inform you about our upcoming 1st CSSB International Symposium
Systems in Infection Biology - From Molecules to Organisms to be held in
Hamburg, 9-11 April 2015. Further information can be found at
www.cssb-symposium2015.de. Following the symposium, we will hold
Dear Dialing,
You can use Rotate/Translate zone (
https://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot/web/docs/coot.html#Rotate_002fTranslate-Zone)
to do that, just clicking twice on the same residue to chose a zone of one.
mvh
Folmer
2015-01-15 14:45 GMT+01:00 Dialing Pretty
Dear All,
If I rebuild a 4-residue peptide, and the first 1 is leu. When I rebuild the
first one, the sidechain of leu occupies the electron density of the first 3
residues. Can you tell me the method to turn the side chain of Leu from
position of the first 3 residues to its sidechain position
Dear all,
We are disposing of an old data collection system comprising a Rigaku RUH3R
rotating anode X-ray generator, a Mar345 image plate detector and Osmic Blue
optics, which was installed in 1999 but is still in working order. If anyone
is interested in it, either as a working setup or as
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