Re: [ccp4bb] anti-hexahistidine antibody

Hi Tomas, Thanks. I think this one is directed at a C-terminal his6-tag. I'm not sure it'll work with an N-terminal tag. Elias From: Tomas Malinauskas Sent: Sunday, January 8, 2017 5:39:59 PM To: Fernandez, Elias J Cc:

[ccp4bb] anti-hexahistidine antibody

Dear All, Is anyone aware of a recombinant system that produces an N-terminal, hexa-histidine-directed Fv antibody fragment that can be used for structural studies (crystallography, SAXS, cryo-EM)? Best regards, Elias

Re: [ccp4bb] anti-hexahistidine antibody

Besides my private reply, a few suggestions that may be relevant to the broader audience: 1) for the purposes of this email I will pretend that relevant patents do not matter (of course they do but it's too complex to get into details) 2) there are quite a few antibodies out there directed

Re: [ccp4bb] anti-hexahistidine antibody

Dear Fernandez et al., maybe this anti-His-tag antibody could be useful: PDB ID 1KTR, its sequence is in the paper https://www.ncbi.nlm.nih.gov/pubmed/?term=12054774 Hope that helps, Tomas On Sun, Jan 8, 2017 at 9:24 PM, Fernandez, Elias J wrote: > Dear All, > > Is anyone aware