Re: [ccp4bb] Non-Cysteine modification with heavy atom

Dear Chandra, A Million ways to die in the West (or when screening for HA derivatives). But this has always worked for me: https://www.cell.com/structure/fulltext/S0969-2126(00)00168-4 Just analyse ability to still form complex after modification. Cheers, Jesko Am Mi., 2. Jan. 2019, 20:42 hat

Re: [ccp4bb] Non-Cysteine modification with heavy atom

Off the top of my head, assuming nothing much about your protein(s) - some of this is only going to work if you can modify the protein(s) prior to crystallization. This excludes the 'normal' heavy atom soaking type stuff which can give rise to binding with covalent-like strength (while still being

[ccp4bb] Non-Cysteine modification with heavy atom

Dear CCP4 members, Can someone suggest to me a methodology to covalently modify (non-cysteine) on component of a multicomponent complex with heavy atoms in order to achieve better phase determination? Thanks in advance Chandra kattamuricha...@yahoo.com

Re: [ccp4bb] Non-Cysteine modification with heavy atom

Hello Chandra, Soaking heavy atoms into crystals has a long and successful history (I use it pretty often and it works much of the time). If you need phase information and you have some crystals, I would certainly give it a try. There are many papers and books to give you methods to do this

Re: [ccp4bb] 2019 New Year’s resolutions of a cryo-EM newbie

Hello Jacob I know it was a bit of a cynical joke and I know it does hurt ... After all my mother's Ellis Island records are dated 1923. Our last family reunion was 15 years ago in Illinois and Missouri. My Mid-West raised uncle was torpedoed off the coast of Cape Canaveral in 1942 transporting

[ccp4bb] Imaging support scientist and software scientist positions at Diamond light Source

Dear CCP community, I’d like to draw your attention to two vacancies we have currently at the X-ray tomography and super resolution fluorescence microscopy beamline B24 at Diamond Light Source. We are looking for a

Re: [ccp4bb] 2019 New Year’s resolutions of a cryo-EM newbie

My apologies for posting this on an open board :) I realize that this is completely off topic - but it is a pretty painful topic that affects us all regardless of academic or industrial affiliation, professional preferences etc. Artem --- do not read below unless you want to :) --- If an

Re: [ccp4bb] 2019 New Year’s resolutions of a cryo-EM newbie

6) I most sincerely hope that, if I stick to my five New Year’s resolutions and stop wasting the cryo-EM community’s time, my fellow Canadians will not extradite me to the fake-news country at our southern border. I know this is a joke, but we in the USA are not a fake news people, and