Hate to self-promote, but these crystals were interesting, At 30deg, two
crystal forms co-existed with
inverted solubility - one form melted when cooled, the other melted upon
heating.
https://www.sciencedirect.com/science/article/abs/pii/S0006349519305879
Dear Sergei,
There is also this high temperature crystallisation study of lysozyme, up to 55
degrees C:-
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2872%2980273-4
Best wishes,
John
Emeritus Professor John R Helliwell DSc
> On 1 Aug 2019, at 10:23, Sergei Strelkov wrote:
>
Dear all -
I have also had success with crystallization above room temperature. I once
grew diffracting crystals of a small, thermostable enzyme by incubating the
plates in a 37°C bacterial culture incubator, and despite similar reservoir
conditions, these high-temperature crystals grew in a
Thank you for that. Good idea to look at the policy. I think it would be
classed as a minor change after release:
https://www.wwpdb.org/documentation/policy#toc_changes
"Update or change on structure factor or constraint file due to format
corrections or addition of data set while coordinates
Temperature is an interesting parameter, but I agree with Janet and others that
it is difficult to predict what will work best.
At the High-Throughput Crystallization Screening Center, we typically run 23C,
14C, or 4C screening experiments to screen for initial crystallization
conditions. We
Hello David
I note that no changes will be allowed to the experimental data, but if there
are no experimental data, will the depositor be allowed to upload it/them,
without starting a new deposition? I'm finding a few reflection files that
ought to be deposited so that the corresponding
Hi Jonathan,
It would be great if you deposit missing reflection data. It is very
commendable. I don't know what the policy is, but I have seen quite a few
legacy entries for which the data were deposited (or corrected) much later than
the model. Sometimes more than 20 years. These kept the
