[ccp4bb] A community support letter for the “UK XFEL” project

[Orville, Allen (DLSLtd,RAL,LSCI)]

To whom it may concern,

To explore the UK and International community appetite for a next generation 
XFEL we are conducting this survey to gather information and sample support for 
the UK XFEL project (which seeks to deliver such next generation capability).

X-ray free electron lasers (XFELS) have emerged as important and wide-ranging 
tools for advanced science over the last decade. Their impact has been 
extensive (from catalysis and energy research to structural biology and 
laboratory astrophysics) and has opened-up a new era in research in structural 
dynamics. In the UK and around the world significant numbers of scientists have 
begun to engage with these opportunities, and many more are hoping to do so as 
the capabilities are developed, and as capacity grows so that it becomes easier 
to secure access.
The UK XFEL Science Case 
https://www.clf.stfc.ac.uk/Pages/UK-XFEL-science-case.aspx
examines the transformative scientific opportunities that will be created with 
a Next Generation XFEL i.e. one that can deliver near transform limited pulses, 
high reproducibility, and synchronised to a wide range of external beams (e.g. 
lasers, THz and electrons) all delivered at a high repetition rate with a pulse 
structure matching state-of-the-art sample delivery and detector technologies. 
We strongly endorse this scientific vision, and fully support the next stage in 
the efforts to ensure that there is an overall capacity increase and that these 
new capabilities can be made available over the next decade for advancing 
science and technology.

The survey link is:
https://forms.gle/KdZ3XefZHt9oNYY26”


Sincerely,

AMO, et al
~ ~ ~ ~ ~
Allen M. Orville, Ph.D.
Wellcome Investigator and Royal Society Wolfson Fellow
Principal Scientist, XFEL Hub at Diamond Light Source
Harwell Science and Innovation Campus
Didcot, Oxfordshire
OX11 0DE
United Kingdom

Phone: +44 (0) 1235 567505
Mobile: +44 (0) 7471 026061
email: allen.orvi...@diamond.ac.uk
Skype: allen.orville
ORCID: -0002-7803-1777
AMO site: https://www.diamond.ac.uk/Instruments/Mx/XFEL-Hub/Staff/Orville.html
XFEL-Hub site: https://www.diamond.ac.uk/Instruments/Mx/XFEL-Hub.html



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[ccp4bb] Post-doctoral position available

[Page,Rebecca]

The Page Laboratory at the University of Connecticut Health Center has an 
immediate opening for up to two post-doctoral candidates to study pro- and 
eukaryotic signaling enzymes, with a focus on the function of phosphatases that 
form large signaling complexes, and also drug/inhibitor development. All 
studies use X-ray crystallography and/or cryo-EM/NMR. Applicants with a strong 
background in biochemical techniques, and especially eukaryotic protein 
expression and protein crystallization, are encouraged to apply.


The Page laboratory is housed in a newly renovated ~250,000 sq. ft. research 
laboratory in Farmington, CT. The laboratories are open-lab-space facilities 
and allow close interaction with the adjunct structural biology and 
molecular/cell biology groups at UConn Health. The Page laboratory is equipped 
with all necessary infrastructure to produce (E. coli, Sf9, mammalian), purify 
and analyze protein and protein complexes (ITC, SPR etc.) and crystallization 
robotics for crystal production.


Farmington, CT, is located on the New England between Boston (90 min.) and New 
York City (90 min.). Starting date, salary and project details are negotiable.

Please e-mail a Cover Letter and CV to Rebecca Page (rpage(at)uchc.edu).


Recent publications:

  *   Wang, X., Garvanska, DH, Nasa, I, Ueki, Y, Zhang G, Kettenbach, AN, Peti, 
W, Nilsson, J, Page, R (2020). A dynamic charge-charge interaction modulates 
PP2A:B56 substrate recruitment. eLife. 9. Pii: e55966.
  *   Choy, M.S., Moon, T.M., Ravindran, R., Bray, J.A., Robinson, L.C., 
Archuleta, T.L., Shi, W., Peti, W., Tatchell, K., Page, R. (2019) SDS22 
selectively recognizes and traps metal-deficient inactive PP1. PNAS 116:  
20472-20481.



Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030
rp...@uchc.edu

Page laboratory website



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[ccp4bb] Post doctoral position in structural biology of ER protein complexes

[Kajander, Tommi A]

Dear All,

I’d like to draw your attention to open post doctoral position in our 
laboratory in Helsinki in biochemical and biophysical characterisation and
structural biology of novel endoplasmic reticulum stress regulator protein 
complexes.

Position is for one year initially with possibility to continuation, if you or 
your colleagues know someone (or are someone) with right skill set might be 
interested please apply/ let them know / feel free to circulate.

Know-how in protein expression and purification/characterisation and preferably 
some experience structural biology is required,
but it will also be an opportunity to learn and utilise multiple techniques in 
biochemical and biophysical characterisation.

We have a very friendly vibrant and interactive scientific community in the 
research program and on campus overall.

The advertisement is available here (applications only via the web site):

https://www2.helsinki.fi/en/open-positions/postdoctoral-researcher-in-structural-biology


Best regards,

Tommi



Tommi Kajander, Ph.D. Adj. Prof.
Institute of Biotechnology
University of Helsinki
Viikinkaari 1 (P.O. Box 65)
00014 Helsinki, Finland
p. +358-2-941-58904
http://www.helsinki.fi/kajanderlab








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[ccp4bb] iUCR-25 takes place in August 2021

[Eugene Krissinel - STFC UKRI]

Dear All,

We would like to draw your attention to the recent announcement from iUCR-25 
organisers:

https://mailchi.mp/909bcb8a8cd9/iucr25-prague-call-for-abstracts?e=e7b950fa73

Last 12 months may be confidently called as the "year online", and we wonder if 
there may be fresh submissions to the "Online crystallography" Special Session 
of iUCR-25: we would gladly consider reports not only on new online tools and 
methods, but also on your experience of doing crystallographic research in 
online and hybrid mode, revealed need and ideas for new online instruments, 
approaches and all related matters. Please contribute and share your thoughts 
and findings.

Many thanks,

Eugene Krissinel
Christian Hübschle
Chairs, Special Session "Online Crystallography" iUCR-25


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Re: [ccp4bb] Co crystalization with less soluble ligand.

[David Briggs]

These are my go-to review articles for ideas for getting a ligand into a 
crystal.

https://scripts.iucr.org/cgi-bin/paper?S0907444906047020

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297911/

I have also seen a talk where someone successfully dissolved their 
water-insoluble ligand in Octan-1-ol, and layered that solution over the drop 
after crystals had grown. They incubated for X period of time (I don't recall) 
froze the crystals and found the ligand had diffused into the crystals and was 
in the active site.

Maybe someone else knows of the/a paper describing this?

Hope this helps,

D


--

Dr David C. Briggs

Senior Laboratory Research Scientist

Signalling and Structural Biology Lab

The Francis Crick Institute

London, UK

==

Diamond User Committee MX representative

==

about.me/david_briggs


From: CCP4 bulletin board  on behalf of Gourab Basu 
Choudhury 
Sent: 24 April 2021 03:46
To: CCP4BB@JISCMAIL.AC.UK 
Subject: [ccp4bb] Co crystalization with less soluble ligand.


External Sender: Use caution.

Hello everyone,

I am finding it difficult for getting a ligand density inside the protein as 
the ligand is very much insoluble. It's only soluble in 100% DMSO. I tried for 
co crystalization. Kd value of the ligand is near 40um. Any suggestion what to 
do?



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The Francis Crick Institute Limited is a registered charity in England and 
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Re: [ccp4bb] Co crystalization with less soluble ligand.

[Bernhard Spingler]

Dear Gourab,
You might just want to add the ligand in pure, solid form to the edge of the 
crystallization drop. This worked in our case beautifully (see Angew. Chem. 
Int. Ed. 2012, 51, 3354). We had to soak it for 4 weeks.
Good luck!
Best regards
Bernhard



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