Re: [ccp4bb] help for motion correction

2022-07-16 Thread Suparno
Hi, Please check the X and Y of your gain reference file using the e2iminfo.py command. It is possible that those values do not match those of the micrographs. If it is indeed that case, you can use the relion_image_handler options (for example --flipXY) to flip the gain reference file. Hope this

Re: [ccp4bb] help for motion correction

2022-07-16 Thread Martyn Winn - STFC UKRI
Might be better posted to cc...@jiscmail.ac.uk m -Original Message- From: CCP4 bulletin board On Behalf Of Jing Sent: 16 July 2022 20:56 To: ccp4bb Subject: [ccp4bb] help for motion correction Hello All, I have a question about gain reference file. When I try to work on Motion

[ccp4bb] help for motion correction

2022-07-16 Thread Jing
Hello All, I have a question about gain reference file. When I try to work on Motion Correction on relion 3.1.3, it always reports an error message said "the size of image and the size of the gain reference do not match." Because of that error, I could not proceed motion correction of my

Re: [ccp4bb] Help with One dimensional electron density calculation

2022-07-16 Thread Pavel Afonine
For some perhaps relevant discussion please see Appendix B here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096492/pdf/d-74-00531.pdf Pavel On Sat, Jul 16, 2022 at 6:17 AM Marcin Wojdyr wrote: > On Thu, Jul 14, 2022 at 3:15 AM Pavel Afonine wrote: > > > Map values are calculated using

Re: [ccp4bb] Help with One dimensional electron density calculation

2022-07-16 Thread Marcin Wojdyr
On Thu, Jul 14, 2022 at 3:15 AM Pavel Afonine wrote: > Map values are calculated using tricubic interpolation. Optionally you can > choose a less accurate eight-point interpolation (use interpolation keyword > for this). > I've been wondering which one is better. Tri-cubic is more accurate