Re: [ccp4bb] TEV vs HRV3C

2022-12-08 Thread Dom Bellini - MRC LMB
Hi All, I agree with everything said and I also find 3C, my first choice, to cleave more efficiently/rapidly. But I remember all the folks working with membrane proteins were using TEV, so I wonder whether TEV may be more resistent to detergent? (but I never read/found any info about this).

[ccp4bb] Structural biology+molecular simulations PhD studentship at Newcastle University

2022-12-08 Thread Paula Salgado
Dear all We have a BBSRC-funded PhD studentship available and would appreciate if you can circulate to your institutions and potential candidates. https://www.findaphd.com/phds/project/piercing-the-armour-c-difficile-s-layer-permeability/?p150321 The project builds on our recent work on C.

Re: [ccp4bb] ligands found in ChEBI...

2022-12-08 Thread Harry Powell
Hi folks it seems that the “easiest” way to get my coords if I know the ChEBI identifier is to do something like this in Python3 (note this snippet has no error checking!): > import sys # only needed to parse the command-line arguments > chebi = sys.argv[1] # expect ChEBI identifier without

[ccp4bb] Two PhD student positions in the lab of Titia Sixma at the Netherlands Cancer Institute, Amsterdam

2022-12-08 Thread Titia Sixma
Two PhD positions in the group of Titia Sixma at the Netherlands Cancer Institute in Amsterdam to study processes at the interface of DNA repair and replication using cryo-EM and quantitative biochemistry (https://www.nki.nl/research/research-groups/titia-sixma/). These are both Marie

[ccp4bb] Doctorate and postdoc positions @Golub lab, Israel

2022-12-08 Thread Eyal Golub
Dear CCP4 Community, We are a new and dynamic group at Bar-Ilan University. We investigate the effects of biological confinement within protein-based structures, i.e. protein cages and nanotubes. Towards that end, we combine state-of-the-art techniques including protein engineering and

[ccp4bb] shipping dewar question

2022-12-08 Thread Thomas, Leonard M.
Hello All, I have had an odd thing happen with one of our CX100 shipping dewars. It became obvious that it had lost its vacuum, cold outside, LN2 boiling off quickly. I pumped the dewar down, vacuum was not great but I figured I would test to see if it was holding vs. warming up immediately.