Dear Sajid --
Thanks for your suggestions to run revise. Suggestions
from Mike Latchem helped me to overcome the problem.
I have another question.
I am trying to run REFMAC5. It is giving some warning
message.
#
There is no file name for parameter MAPOUT1
The CCP4 program
This might help...
Hassell AM, An G, Bledsoe RK, Bynum JM, Carter HL 3rd, Deng SJ, Gampe
RT, Grisard TE, Madauss KP, Nolte RT, Rocque WJ, Wang L, Weaver KL,
Williams SP, Wisely GB, Xu R, Shewchuk LM.
Abstract
Crystallization of protein-ligand complexes.
Acta Crystallogr D Biol Crystallogr. 2007 Ja
Dear Jiamu Du --
I want to crystallize a mAb Fab in complex with a hydrophobic
cyclic peptide. The peptide contains 1 hydrophilic residue, 2 Cys,
and 9 hydrophobic residues. The problem is that I can not dissolve
this peptide in common buffers. I think it is need to dissolve it
in some or
Dear All
Thanks for your suggestions to run revise. Suggestions
from Mike Latchem helped me to overcome the problem.
I have another question.
I am trying to run REFMAC5. It is giving some warning
message.
#
There is no file name for parameter MAPOUT1
The CCP4 program is la
Dear Ngo,
You could try additive screens, such as those sold by Hampton Research.
Using an additive identified during screening, I was able to optimize
conditions giving thin, fiber-like needles to get chunky plates. This was
with a soluble protein, though. I don't know whether this approach will
Dear all,
sorry for the off-topic question. I am looking for an affordable+small
crystallisation incubator (16-21 deg Celsius). Ideally, it would fit
neatly under my bench (in a genetics lab) and hold a few dozen 96 and
some 24 well plates. A friend already suggested to get a wine-cooler?!
I
Dear All
what are the experiences in the community in terms of
physico-chemical property changes if one replace a S-Methionine by a
Se-Methionine?
Are there structural a/o functional changes known, e.g. the wild type
protein binds a ligand whereas the Se-Met version does not?
tha
DDM does not form crystals in aqueous solution. The published phase
diagrams show this. In fact, few of the alkyl glycoside detergents
crystallize if the sugar group is a beta anomer, even in organic
solvents. This is one of the reasons why they are so difficult to
purify by recrystalliz
Hi Ngo,
Obviously, you can spend years optimizing crystallization conditions for
a membrane protein. Or, you may get lucky and do it quickly. In any
case, I would recommend to try, in parallel, collecting data on one of
the micro-diffraction beamlines. We offer such capability at GM/CA-CAT
beamli
hi Ngo,
Reduce such showering by increasing the reservoir volume, or by increasing
the protein concentration.
On Wed, Apr 16, 2008 at 4:08 PM, Van Den Berg, Bert <
[EMAIL PROTECTED]> wrote:
> Hi Ngo,
>
> your needles actually look like very thin plates. They seem promising to
> me. From appeara
Hi Ngo,
your needles actually look like very thin plates. They seem promising to me.
From appearance its impossible to tell whether the crystals are detergent or
not. DDM is apparently known to form crystals, but I've never really gotten any
(DDM is very soluble). What temperature have you use
You may be able to check whether the cyrstals are protein or detergent
this using powder diffraction. (please excuse the shameless plug):
Acta Cryst. (2008). A64, 169-180 "Powder crystallography on macromolecules"
I. Margiolaki and J. P. Wright
http://journals.iucr.org/a/issues/2008/01/00/sc5011
You didn't mention which detergent in the conditions. From the picture it looks
like crystals of detergent.
--
Hubing Lou
Centre for Biomolecular Sciences
University of St Andrews
North Haugh
St Andrews
Fife
KY16 9ST
Scotland
Quoting Ngo Duc Tri <[EMAIL PROTECTED]>:
> Dear ccp4 users,
> I cry
Deutsches Elektronen-Synchrotron
A Research Centre of the Helmholtz Association
DESY is one of the world's leading accelerator centres for investigating
the structure of matter. DESY develops, builds and operates large
accelerator facilities for photon science and particle physics. DESY
looks bac
Dear Sajid
It sound as though you are running Revise through CCP4i. If you do this,
the first thing you normally select is your mtz file. The buttons
labelled 'FPH+1' and 'FPH-1' in the gui then default to the same thing.
You need to click on at least one of these buttons and select a
different la
Dear All,
I want to crystallize a mAb Fab in complex with a hydrophobic cyclic
peptide. The peptide contains 1 hydrophilic residue, 2 Cys, and 9
hydrophobic residues. The problem is that I can not dissolve this peptide in
common buffers. I think it is need to dissolve it in some organic solvent,
su
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