Hi,
can you perform a thermal shift assay ? Google or it or find some
references in a posting not too long ago perhaps 6 months or less.
If so you can check your protein with various additives and see which
ones stabilize your protein.
What happens if you dialyse your sample instead of
I would try the following:
1. Remove the His tag.
2. If you need to get rid of the imidazole I would try adding ammonium
sulfate.
3. Is your protein proline- and/or rich in aromatic residues? This may
explain your need for imidazole and solubility.
Hi there,
nbsp;
Sorry for the off topic
Hi Neeraj,
a couple of S100 proteins bind Zn2+ and Ca2+. Ca2+ binds to EF-hands;
Zn2+ binds to specific sites distinct of the EF-hands. We are
investigating Zn2+-binding by spectroscopy and crystallography. We think
that Zn2+ is like Ca2+ a kind of second messenger. If you have
questions, I
Thank you Ian for the comment !
Apparently, I was a bit too quick in my answer.
By the way, my mentioning of Fresnel's theory was of pure historical
interest and not at all to say that the whole story was written at that
time.
I went back to my Born Wolf (some kind of a bible in the optics
Hi,John,
I faced the similar situation before even the resolution about 2.4. At
that region the residues are all hydrophobic ones and should be very
flexible . Arp/warp and phenix do not work well and resolve does not
improve the density either. At the end I just try to build the model
as
Dear Victor
I'm glad that you see the desirability of updating Arp/warp to allow
any setting. I'm a recent convert to this idea myself
Best wishes
Phil
On 11 Jun 2008, at 09:17, [EMAIL PROTECTED] wrote:
Thanks very much to everybody for useful discussion on space groups,
axes and
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Ethan Merritt
Sent: 12 June 2008 19:41
To: Multiple recipients
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] birefringent spacegroups
But the ellipsoid is only a convenient approximation based
The CCP4 courses page includes a variety of talks, tutorials and (most
importantly!) photos from past events, see
http://www.ccp4.ac.uk/ccp4course.php
under Some documentation of past events
In particular, I've put the MR tutorial from Oulu under the entry
BIOXHIT/BCO course on CCP4 structure
PS I found a nice presentation from the NIST group explaining the theory
behind the cubic birefringence effect:
http://math.nist.gov/mcsd/Seminars/2004/2004-06-10-shirley-presentation.
pdf . Slide 33 has some nice graphics summarising the birefringence
effect in all crystal systems.
-- Ian
G'day,
Given my newbie status (and paucity of material on the subject), would
everybody be so kind as to share their experiences with crystallization of high
salt protein samples (I'm thinking 0.3M NaCl or greater).
n.b. googling salting out protein crystal gives a year 2000 summary posting,
You shouldn't be trying to install clipper or fftw.
If you use the precompiled coot binaries, then you get precompiled
clipper and fftw as part of the package.
If you want to build it yourself (not recommended except for experts),
you should use one of the autobuild scripts which will
I just graduated, but I didn't look very hard for experimental
crystallography positions, so I can't comment on job ads. Still, I live in
Berkeley, which gives me plenty of perspective on the state of the field.
Two thoughts:
1. Many universities would prefer not to hire many tenured professors
Concanavalin A can be made to bind Zn and Ca. Its binding site has room for
one transition metal and one alkaline earth metal.
http://www.jbc.org/cgi/content/abstract/271/27/16144
Highly recommended reading material on one of the classical protein targets
Sumner's Nobel Prize lecture is here
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