I can only say that I've purified dozens of metalloproteins using IMAC and
have so far not noticed many issues with metal ion losses. Having said this,
I also must add that I've been exclusively using His-SELECT resin
(unintentional but well-deserved plug for Sigma) for the past few years, and
ther
Dear Crystallographers,
Does anybody happen to know whether imidazole is able to chelate metal ions in
solution? It seems reasonable that since it can compete for binding to IMAC
resins, it should have some affinity for at least Ni++ and Co++, but what about
metal ions like Ca++ and Mg++? I ass
Hello all,
I am trying to put together a list of available beamlines that now do either
remote data collection or "mail-in" collection.
So far I've found:
BNL "mail-in" facility
SSRL all PX beamlines have remote capability
ALS 4.2.2 beamline has remote capability
Any input/corrections would be wel
Dear all,
Thank you so much for all the helpful replies. I took the advice to copy
the Lsqkab output into excel and then make the plot.
The tricky thing is that, by doing the copy/paste, not only numbers will be
copied, but also the text next to it. Immediately after pasting into excel,
each ce
Dear All
I want to run MrBump. We have dynamic internet system in our institute. So I am
not able to locate the proxy address.
Any help please
Sajid
From Chandigarh to Chennai - find friends all over India. Go to
http://in.promos.yahoo.com/groups/citygroups/
On Thursday 17 July 2008 09:48:08 Shawn Leeds wrote:
> Dear all,
>
> I am trying to generate 2D Ca deviation plots for my superimposed molecules
> as needed for publication. I am wondering what is the program of choice for
> such a purpose? I tried Lsqkab in CCP4, but wasn't sure what's the most
Dear all,
I am trying to generate 2D Ca deviation plots for my superimposed molecules
as needed for publication. I am wondering what is the program of choice for
such a purpose? I tried Lsqkab in CCP4, but wasn't sure what's the most
efficient way of generating good quality 2D deviations plots f
CCP4 bulletin board wrote on 07/17/2008 11:25:20
AM:
> Dear all,
>
> I have a request for a different kind of paper. Does anyone know of
> a supplier of the X-ray sensitive "pink" (or green) paper used to
> locate X-ray beams.
>
> James
Hi James -
I've used Gafchromic film (www.gafchromic.com
Hi -
I would try SHARP; or to be exact be tempted to think less and use
autoSHARP with the description of all datasets and only give it the Se
sites for the seMet crystal. The same thing can also be done easily in
Solve. In both cases I would start with unmerged data and let these
program
Dear all,
I have a request for a different kind of paper. Does anyone know of a supplier
of the X-ray sensitive "pink" (or green) paper used to locate X-ray beams.
James
--
Dr. James Murray
PX group
SGC Oxford
For the SeMet dataset, which wavelength did you collect first? If it is the
peak, you could try doing SAD with just the peak wavelength. Maybe combine the
Se peak data with the Hg dataset (provided they are isomorphous) and do MIRAS
as Jacob suggested.
Bert van den Berg
University of Massachus
Wouldn't it make sense to do MIRAS with both the Se and Hg derivatives, and add
in some multi-crystal averaging from the native set? You seem like you are
almost there, though. I assume you have already tried DM and NCS averaging (if
there is any)?
Jacob
***
Hi,
I have a very good native data set. However, the selenomethionyl
crystal has a different space group and always suffers from radiation
damage. A three-wavelength data set was still collected, and after
phasing in SHARP, some features can be seen but the map is not good
enough for trac
In a vain attempt to head off another flurry of emails . can I state
that CCP4 does not condone illegal file sharing, and breaking of
copyright. To avoid any misunderstandings, please do not use ccp4bb to
ask for PDFs of articles. You can usually ask the author in private.
Regards
Martyn
On T
Hi everyone:
Could anyone tell me the meaning of Fbar in the MADFBARFILE in solve? Does Fbar
stand for delta f prime or just f prime for heavy atom?
By the way, I want to learn more about how solve work, but I have no right to
access papers about solve. could anyone send me pdf copies of follow
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