Re: [ccp4bb] creating alternate conformations using PyMol

2008-09-29 Thread Chavas Leo
Dear Neeraj -- On 26 Sep 2008, at 22:48, Neeraj wrote: Is there an easy way to do this in pymol which will help me rotate only a small fragment of the protein while keeping everything else static. What you can do is to generate two coordinate files, one without your loop, one with, and

[ccp4bb] Call for proposals: SLS PX beamlines

2008-09-29 Thread Stefan Mueller
Dear SLS users, Call is open for proposals for the PX (Protein Crystallography) beamlines of the Swiss Light Source, SLS. Deadline for porposal submission: Wednesday, October 15, 2008 Submission: All submissions will be handled by the SLS Digital Users Office (DUO) /contact: [EMAIL

[ccp4bb] BEAMTIME AT SLS X06DA

2008-09-29 Thread Meitian Wang
= SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT THE SUPERBENDING MAGNET BEAMLINE X06DA AT SLS = Deadline: Wednesday, October 15, 2008 Periods: January

[ccp4bb] map and model do not fit when using Turbo-Frodo

2008-09-29 Thread Ben Ammar Youssef
Dear All, I am refining a structure of Se-protein solved with MAD using phenix. The unit cell is P 21 21 21 with a=36.898, b=59.450, c=393.909 and alpha=beta=gamma=90. There are two molecules in A.U. The problem is that when I use Turbo-Frodo for molecular modeling, the map does not fit to

[ccp4bb] program to evaluate proteins

2008-09-29 Thread Jenny
Hi, Dear All, Does anyone have suggestions about what good programs are available that could be used to evaluate proteins?Like how good is the packing, hydrogen bonding pattern, aggregation prediction, solubility, hydrophobic surface burial etc. Thank you very much. Jenny

[ccp4bb] biotech position

2008-09-29 Thread Marius Schmidt
Please bring the following ad to the attention of possible candidates; Best M. Schmidt Position Description: FACULTY POSITIONS IN BIOTECHNOLOGY The University of Wisconsin-Milwaukee invites applications for 2 faculty positions in biotechnology at the Assistant, Associate, or Full Professor

[ccp4bb] cryo-protection for crystals grown in ethanol

2008-09-29 Thread Rongjin Guan
Dear All I got crystals from 20% Ethanol with 0.1M Tris pH 8.5. This is my first time to have crystals in Ethanol and want to get some suggestions of cryo-protection from those who have done this before. I am waiting for my time on home X-ray facility, and hope I can get some suggestions before

Re: [ccp4bb] cryo-protection for crystals grown in ethanol

2008-09-29 Thread Katya Heldwein
Hi Rongjin, I had crystals that grew in 9% ethanol as a precipitant. To cryoprotect them, I first replaced the mother liquor with solution containing 10% MPD, instead of ethanol, and then briefly dunked the crystals into ~30% MPD. Hope this helps. Good luck. Katya On Mon, Sep 29, 2008 at 1:24

[ccp4bb] CCP4i can NOT run

2008-09-29 Thread Yingjie Peng
Dear friends, I have installed the latest version of CCP4i under Fedora 9 on my laptop HP Compaq 6515b (two processors). The system setup was done and the CCP4i interface can run normally. But if I submit a job, it displayes STARTING the job in the job window and will NOT actually run the job.