Hi,
you could try without heating. Just vortex your sample a few times after
adding the sample buffer and then load the gel.
good luck,
Daniela
> Is your phosphate a Na-K phosphate? If it is, then you're probably getting
> a
> K salt of SDS which is considerably less soluble than Na or Li sal
Now that everything with coot and imosflm has been settled, how about
sftools? I am getting errors when I try to open an sftools window with
ccp4i, with 6.1.0. I get the same error with a fink-installed mac
version, and a linux installation. Here is the error:
bad window path name ".main.ca
Besides the K-SDS precipitation it can also be the problem of membrane
proteins generally having a tendency to aggregate when boiled with
SDS. Try to just add the Laemmli buffer and load the gel without
boiling. This is the general procedure for membrane proteins.
Poul
On 18/12/2008, at 0
Is your phosphate a Na-K phosphate? If it is, then you're probably getting a
K salt of SDS which is considerably less soluble than Na or Li salts.
Artem
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Subscribe Ccp4Bb Kn L
Sent: Wednesday, December
Hi all,
puzzled by an increase of R-factors when switching to the new ccp4 and
along with it from refmac 5.5.0063 to 5.5.066, I took a closer look.
Same refinement, same input (in fact, started from the interface with
re-run job) gives distinctly different statistics. It appears to be
only
Hello everyone...
I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it aggregates (as in when you mix GuHCl with SDS) and can't be
loaded onto the gel.
Does anyone know why it happens? Thanks a lot
Hi
After all v6.1.0 did arrive before Christmas :-) Thank you all.
Still I guess Santa, after freezing in the car park, still had to
endure beeing stuck in some chimney, as a consequence of all of us who
wanted to grab a fresh copy, flooding poor CCP4 server. So, after 2
days I fin
Hi Jon,
- obviously, the model should not be manipulated once the final
statistics is obtained, or if it is manipulated, then the final
statistics must be re-calculated;
- I can't imagine someone thinking that hydrogens were refined
individually in a X-ray structure say at 2A resolution.
-
On Wednesday 17 December 2008 15:53:42 Jonathan Winger wrote:
> Hi all-
>
> Just wondering what the consensus is - should a structure refined with
> riding hydrogens be deposited with the coordinates for the hydrogens
> included? One could argue that, since they were used in the
> refinemen
Yes, William I do agree with you,
and in the same line of doubt, I have one more thing to ask you.
Where is this .cvspass resides.
I have problem in updating my fink , with fink selfupdate-cvs.
thanks
S.Jayashankar
Research Student
Institute for Biophysical Chemistry
Hannover Medical School
Ger
Hi all-
Just wondering what the consensus is - should a structure refined with
riding hydrogens be deposited with the coordinates for the hydrogens
included? One could argue that, since they were used in the
refinement to obtain the model and statistics reported, they should be
included
Please, we all need to mention this to the Apple people. This is
totally unacceptable.
On Dec 17, 2008, at 2:54 PM, Engin Ozkan wrote:
Yep, this happened because of the latest Mac OS 10.5.6 update. With
the update, my X11 version got downgraded from 2.3.1 to 2.1.5.
Engin
William G. Scot
On Wednesday 17 December 2008 14:26:32 Eugenio De la Mora wrote:
> Hi,
> I have some XAS spectra but I'm new in the area. I'm looking for a free
> software. I just want to normalize the data and compare between them. Any
> suggestion?
http://depts.washington.edu/uwxafs/
--
Ethan A Merritt
Bi
Hi Engin:
What do you get in response to the command
otool -L /usr/X11/lib/libXdamage.1.dylib | grep "libXdamage.1.dylib"
You should see
/usr/X11/lib/libXdamage.1.dylib (compatibility version 3.0.0,
current version 3.0.0)
If you don't, update X11 here (free) to at least 2.3.1:
http://xqu
Yep, this happened because of the latest Mac OS 10.5.6 update. With the
update, my X11 version got downgraded from 2.3.1 to 2.1.5.
Engin
William G. Scott wrote:
Hi Engin:
What do you get in response to the command
otool -L /usr/X11/lib/libXdamage.1.dylib | grep "libXdamage.1.dylib"
You shou
Hi,
I have some XAS spectra but I'm new in the area. I'm looking for a free
software. I just want to normalize the data and compare between them. Any
suggestion?
Thank you very much.
Eugenio De la Mora
Insituto de Biotecnologia, UNAM.
By the way, is anyone experiencing the following problem with
fink-installed coot?
dyld: Library not loaded: /usr/X11/lib/libXdamage.1.dylib
Referenced from: /sw/bin/coot
Reason: Incompatible library version: coot requires version 3.0.0 or
later, but libXdamage.1.dylib provides version 2.0.0
Hi
I should say, while this subject has been brought up, that Luke and I
are working hard to remove many of these dependencies, so iMosflm
should work with a more "plain vanilla" version of TclTk (but you will
still need iTcl and iTk.
On 17 Dec 2008, at 16:34, William G. Scott wrote:
Mo
More specifically, issue
fink selfupdate-cvs (or fink selfupdate-rsync)
fink install itcl itk iwidgets tdom tkimg tktreectrl
On Dec 17, 2008, at 7:56 AM, Andrzej Lyskowski wrote:
Hi,
I've just upgraded my fink distro and was wondering what else has to
be done concerning configuration i
I've just now added these as fink packages (cvs unstable). Sorry for
the delay. I dropped the ball.
Steffen Schmidt did the hard work, so many thanks to Seffen!!!
Bill
On Dec 17, 2008, at 7:56 AM, Andrzej Lyskowski wrote:
Hi,
I've just upgraded my fink distro and was wondering what els
I can only recommend the Motic microscopes. i'm very impressed for $1,70O incl
camera, not as sturdy as Leica (my favorite) but very good image quality.
Drawback: no cold light source. But you can get a gooseneck light and do top
illumination. Do not buy their polarizer, though.
Cheers,
Jens
Hi Andrzej,
To get iMosflm working you need a few extensions which it uses to be
available. A 'full-fat' Tcl/Tk with everything added which will work for
both CCP4i and iMosflm can be found at
ftp://ftp.ccp4.ac.uk/ccp4/current/extras
(Tcl-Tk++-osx-universal.dmg.gz)
This will install by default
Hi,
I've just upgraded my fink distro and was wondering what else has to
be done concerning configuration in order to make the iMosflm run on Mac OS.
So far I'm getting the following error:
Error in startup script: unknown namespace in import pattern "itcl::*"
while executing
"namespace i
Dear Clint,
Jerome is right. Do not expect to get a stereo microscope (these are
more or less two microscopes within a smart arrangement) for this price.
We purchased recently a Leica M205A, which defines the quality and price
for a high-end system. It has a magnification range using a 0.8 plan
Dear All,
Does anybody has any comment on using heparin as an additive to
co-crystallise an RNA-binding protein? I am not asking about using it
to form a complex with proteins that are know to bind heparin
specifically, but using it as a general RNA analogue, when we lack the
knowledge of th
The combination of for example 10-120x stereo-zoom magnification, good
optical quality, bright- and dark-field illumination,
and only 3000 US$ is NOT possible unless:
1) you are willing to buy a second hand of quality brands like Leica,
Zeiss, Olympus or Nikon (my preference)
2) you buy a low q
I would like to draw your attention to the following opening:
Vacancy Reference 3010245
Applications are invited for a Post-doctoral position funded by Cancer
Research UK, to work on structure/function studies of the NMD
machinery in Dr Laura Spagnolo's group. Applicants must hold a PhD in
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