[ccp4bb] weak magnet of goniometer of MAR345dtb

Hi, We have recently installed a new home source diffraction system using MAR 345 dtb. It works much better than the rigaku because this new device has automatic motor control system. However, we have a lot of trouble to mount crystals. The magnet of MAR goniometer is significantly

Re: [ccp4bb] Lipid Removal from Proteins

I dug around on the net and found this method to remove lipids from proteins: More precisely, from denatured proteins. That's what methanol/chloroform phase does for most proteins. Wessel Fluegge (1984), Anal. Biochem. 138:141-143. Itґs a methanol/ chloroform precipitation and gives you a

Re: [ccp4bb] weak magnet of goniometer of MAR345dtb

Quoting aidong a...@xmu.edu.cn: Hi, We have recently installed a new home source diffraction system using MAR 345 dtb. It works much better than the rigaku because this new device has automatic motor control system. However, we have a lot of trouble to mount crystals. The magnet of MAR

Re: [ccp4bb] Is there Windows version of O ?

On Thu, 27 Aug 2009, lei feng wrote: maybe a little offtopic, but I really need anotehr model buiding software that can run on windows system ( I already have coot) May I ask why you need another modelling tool if you don't even know it?   I found Alwyn Jones 's website, which has version 12

[ccp4bb] Conference Membranes and Modules in Berlin

Dear crystallographers, we are organising a conference Membranes and Modules which takes place in Berlin from December 10 - 13, 2009. It covers a number of topics related to structural biology, macromolecular complexes, biophysics and biochemistry of membranes/membrane proteins and cellular

Re: [ccp4bb] Arpwarp not recognised by latest version of ccp4

Hi - There is no specific reason, to my knowledge, why ARP/wARP will not be recognized by the latest version of ccp4i. At worse, you can re-install the GUI from 'Install Task' from the ccp4i, which should still work. If you have any specific trouble/error messages, please do let me know.

Re: [ccp4bb] Is there Windows version of O ?

Apropos to installing windows crystallography programs: Re: [ccp4bb] Computer hardware and OS survey Jon Wright Fri, 01 May 2009 10:24:28 -0700 Link,Todd M wrote: ... I did not find an equal web support page for Windows. It just isn't needed. If there is a windows version of a program you

Re: [ccp4bb] Lipid Removal from Proteins

James, Dima is right. One of the protocols for protein-refolding with sarcosyl uses cyclodextrins to remove it. However, sarcosyl is an anionic detergent above pH 5.5 under most conditions, so any ion exchange platform should work well to markedly reduce its presence in a preparation.

[ccp4bb] Postdoctoral position, Karolinska Institute, Sweden

*Postdoctoral position in molecular and structural biology of protein complexes in nerve development and disease* Dept. of Medical Biochemistry and Biophysics, Karolinska Institute A postdoctoral position is available immediately in the Molecular Structural Biology group at the Karolinska

[ccp4bb] Rfactor got stuck with a data having alternate strong and weak reflections.

Dear all: I am currently refining a structure solved by MAD and somehow the R factor got stuck around 30% with 2.2 resolution. There are four molecules in one ASU. Two had very good density map and the other two were not equally good. I tried using NCS during

[ccp4bb] Problem with Coot reading monomer library file.

Dear Crystallographers, I've been spending 10 hours trying (googling, manually editting cif files based on templates in Coot's library, asking around, rtfm and reading this bbs) to figure out why Coot the geometric restraints wouldn't load. The molecule I have is difluoroacetate (and some