To avoid the creation of a cumbersome new unit everyone will need to
keep track of, can we just come up with a prefix that means 0.013 of
something? Perhaps we could give it the symbol b and then we could
say the B-factor is 20 bA^2.*
James
*Seemed like 76.92 b humor units when I wrote
Maybe... Depending on the energetics - it could stall the translation -
or prevent initiation if it is at the start of the ORF. If it is in the
middle - you might be ok... Usually the gene synthesis company can
recommend alternate sequences that do not run into this problem or rare
codon
Dear Sangeetha,
Can I assume that you detected the hairpin by computational means? In
general a single hairpin may not necessarily doom your experiment or reduce
expression - however you could reduce the hairpin stability via silent codon
changes just to be on the safe side. Keep in mind that
This question by the Mad Scientist (here after the MS) has provoked
me to give the topic a lot of thought. I think I can provide some
direction towards the solution, but I'm not adept enough with The
Optical Principles of the Diffraction of X-rays (Which people on this
BB should refer to
