Hi everyone, I am preparing a crystallization manual for our group, however,
I found that it is very difficult to distinguish microcrystalline,
quasicrystalline and precipitation, especially when the precipitation was
shiny, like the grit on the beach. Is there a way to distinguish the three?
Whether the shiny precipitate can be redissolved or not is an important
observation. If not: bad. If yes: good
Also one can check if the shiny precipitate can be used for streak-seeding in
new drops. If not: bad/neutral. It yes: good.
A bad shiny precipitate also often forms a large connected
What about X-ray screening, in situ? We do that on BM30A at the ESRF
with the G-Rob
(http://www.esrf.eu/UsersAndScience/Experiments/CRG/BM30A/description_fip/Experiment/robots/dropx).
With crystalline precipitate we observe rings at low resolution.
JL
On 06/10/2010 08:06 AM, joybeiyang wrote:
Dear Joy
- microcrystalline in most cases would be birefringent - this is
difficult to observe these days in our plastic boxed crystallization world with
colours everywhere.
But if you really want to know you can pipette the droplet onto a glass
coverslip and dim the room
Hi Joy,
Yes, I agree that it is very difficult to distinguish those and it is even more
difficult to put it in words or in writing: experts have been talking about
shininess, transparency (rather than brown colour) and birefringence as far as
I know. All these methods have some grain of truth
Dear All
can people confirm that the failing cif files only have one monomer description
in them. I have one example, but that contains two monomer descriptions.
Remove one of the and coot successfully reads the file.
Charles Ballard
CCP4
On 9 Jun 2010, at 14:34, Yahui Yan wrote:
Hello
Sorry this is off topic.
I am trying to push a data set into the corners of the detectors as Mean I/SIGI looks strong for the circle that the
XDS integrates to. The resolution appears to be hard coded in the detector header (and is reported back by XDS in the
.LP files) which seems to override
Dear Nick,
In XDS.INP, set
TRUSTED_REGION=0.0 1.41
This will integrate to sqrt(2) * radius of the inscribed circle,
provided that the resolution ranges are set appropriately.
Best wishes,
Graeme
On 10 June 2010 12:09, Nicholas Keep n.k...@mail.cryst.bbk.ac.uk wrote:
Sorry this is off topic.
Thanks for the many responses. I had been setting the parameters right. The problem was I was not telling xds to go
back as far as INIT in the reprocessing. JOB ALL was the answer
Best wishes
Nick
--
Prof Nicholas H. Keep
Executive Dean of School of Science
Professor of Biomolecular Science
Postdoc and Ph.D Positions available in GPCR research filed at PSI
Two postdoc and one Ph.D positions are open in the field of GPCR
structure and signalling in Biomolecular Research Laboratory Laboratory
(BMR) at the Paul Scherrer Institute, Switzerland. These projects are
funded by our external
This very rapid reply to my withdrawal as a reviewer for NPG journals contains
a response issued last night purporting to clarify issues obfuscated by the UC
Digital Library in their letter. They claim that the data cited by the UCDL are
misleading. I can neither verify or refute this claim.
http://www.theinquirer.net/inquirer/news/1653487/panasonic-ship-152-inch-3d-tv
Panasonic will ship its 152-inch 3D TV this year
...
resolution of 4096x2160 ...
$500,000 price tag ...
They don't say which 3D tech is utilised, I wonder if it requires active
goggles.
--
A PhD studentship is available immediately in my laboratory at the
University of Edinburgh.
Our studies focus on structural and biochemical studies of
macromolecular complexes involved in the maintenance of genome
stability. For more details, please refer to our website:
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