[ccp4bb] Difference map

Hi Folks I have a query regarding the difference map between the two structures ligand bound data (2.5A) and native (2.8A). I tried to calculate the fourier difference map between two data sets ligand bound- native. The protocol in CCP4 that i used is as: 1.merge the mtz file of native nad ligand

Re: [ccp4bb] Difference map

Hi Intekhab Alam, an Fobs-Fobs' map usually works only if the two crystals are isomorphous, which means that there are neither large cell constant changes nor any other larger structural changes (like overall rotations, domain movements, other rearrangements) than the bound compound (ligand,

[ccp4bb] The color of GFP crystals?

Dear CCP4 community, I wonder if anybody has experience about the color of GFP crystals. Is there any chance that the GFP crystal will become colorless? We are working on one membrane protein fused with GFP. Since we got some crystals which didn't show any color (Condition 01), I am not sure if I

Re: [ccp4bb] Difference map

That seems the right procedure. I presume the two crystals have similar cell, etc? What is the Riso plot from scaleit look like - if it is 55% + then there is no isomorphism, but if it is 30% then the map should be reasonable. which phase did you use for the map calculation? Eleanor

Re: [ccp4bb] The color of GFP crystals?

Hi Tri, To me, the first 3 pictures suggest that there is a faint greenish tinge in the little shapes (hopefully crystalline) there. I guess the idea of the Topaz chip is that you can take it as is to the x-ray beam or am I wrong?      Cheers,               Boaz - Original Message -

[ccp4bb] Expressions of Interest in Diamond’s Phase III Beamline proposals

Dear Everyone, As an MX representative of the Diamond User Committee we are writing to remind you that the deadline is fast approaching for Expressions of Interest in Diamond’s Phase III Beamline proposals, three of which are aimed at MX. The three relevant beamlines and their representatives are

Re: [ccp4bb] The color of GFP crystals?

What GFP did you use, your pH is 4.6 or something around there according to your picture. If the pH is true GFP will be colorless. You certainly have some more GFP lying around (without your protein fused to it), just titrate it and see what pH dependency you have. Jürgen - Jürgen Bosch Johns

Re: [ccp4bb] Micromatrix seeding using the mosquito

Not with membrane proteins, however we use our Mosquito with 20 nl seeding standard. Strip 1 Protein, Strip 2 Seed stock solution We first copy the plate by either picking up protein, then reservoir and dispensing together 200-400 nl or we multidispense protein and add the reservoir later

[ccp4bb] Postdoctoral Position in Structural Biology lab

Postdoctoral candidates: Postdoctoral Position in Membrane Transport The Pinkett laboratory at Northwestern University is seeking a highly motivated individual for a postdoctoral fellow position in the field of protein structural biology. The individual will join a research team studying the

Re: [ccp4bb] The color of GFP crystals?

Dear experts, Thank you very much for your input. Here are my answers for your questions: To me, the first 3 pictures suggest that there is a faint greenish tinge in the little shapes (hopefully crystalline) there. I guess the idea of the Topaz chip is that you can take it as is to the x-ray

[ccp4bb] refmac 5.6 and the ccp4i task interface

Is it possible to use refmac 5.6 with the ccp4i Refmac5 task? Can I just copy the 5.6 binary over the 5.5 binary distributed with CCP4? It looks like the new refmac has a number of new keywords which wouldn't be accessible doing this, but would it otherwise be compatible? If so, where does the

Re: [ccp4bb] ? steps after detwinning

@R.Brown I'm using DENZO I'm trying POINTLESS but I don't understand unmerged data -what's that? is it the spot images which are used for peak search during data processing? Both SFCHECK phenix.xtriage showed the information about pst twinning. However I've tried a thorough process of

Re: [ccp4bb] refmac 5.6 and the ccp4i task interface

Yes. There are two ways: 1) replace $CBIN/refmac5 with refmac5.6 2) on ccp4i click System administration, select configure interface and jus below Give full path name for CCP4 programs to overcome name conflicts click Add a program There will appear two fields. On the right field type refmac5

Re: [ccp4bb] The color of GFP crystals?

In my hands GFP fusions can produce colorless crystals (but still have GFP!) depending on the condition. Sometimes the crystals gradually become colorless with time - bleaching or perhaps some chemical effect - no idea. I've not tested the colorless crystals with UV to see if they still glow.

[ccp4bb] Seeking Postdoctoral Researcher

Laboratory: Zhang Initiative Research Unit, Advanced Science Institute, RIKEN, Japan. (Unit Leader: Dr. Kam Zhang) Job title and Job description: Postdoctoral Researchers Job description: We are seeking a highly motivated and experienced computational structural biologist to join the Zhang