Re: [ccp4bb] hydrohyapatite column
Hydroxyapatite can be miraculous, but is often very capricious: among others, it is temperature sensitive and two different corners of a somewhat inhomogeneous cold room may provide different results. Daniel Le 29/11/2010 16:08, Sebastiano Pasqualato a écrit : Hi all, I read/heard that hydroxyapatite column can be used to purify proteins, getting separation results orthogonal to ion exchange and size exclusion chromatography. I was wondering if any of you would be kind enough to share her/his experience with me, and would suggest vendors and models for such columns. Thanks in advance, best, s
[ccp4bb] Postdoc position at SLS
Postdoctoral Fellow Your task You are responsible for the development of an extension of the fast global electron beam orbit feedback system, with the aim to stabilize the X-ray beam in position and angle at the position of the sample over a wide frequency range. To this end you will characterize the dynamic response properties of the electron beam and X-ray beam position monitors. Based on these results, beam dynamics studies will be carried out to determine the influence of the envisaged feedback system extension on the global orbit stability. The system will require a completely transparent X-ray optical system. Automatic initialization, self calibration and analytical modeling of the beam- transport by means of geometrical optics is hence a prerequisite. Feedback interlocks will be necessary in order to minimize the risk of accidental orbit distortions or even beam dumps due to the system.Since your contribution is most critical for the operation of the SLS you will have to design, implement and test the feedback system extension in close collaboration with the responsible beam dynamics and diagnostic experts as well as the corresponding beam line scientists. Your profile You hold a PhD degree in physics, with experience in theoretical and experimental accelerator physics in the fields of beam optics or in the field of X-ray optics. You are a skilled user of common related software tools (e.g. MAD, Elegant, MATLAB, XOP, SRW) and a programming language, ideally C/C++. You have basic knowledge of electronics and related measurement techniques. Experience in simulation of X-ray beam lines is advantageous. You enjoy the work in an interdisciplinary and multi-national research team. You are motivated to expand your knowledge and experience and look forward to the development of novel feedback systems exploiting synergies between the accelerator and the beam line domain. Good communication skills in English are expected, knowledge of German is advantageous. For further information please contact Dr Michael Boege, phone +41 56 310 45 88,michael.bo...@psi.ch or Dr Meitian Wang, phone +41 56 310 4175 ,meitian.w...@psi.ch Please submit your application (including CV, list of publications, statement of research interests and addresses of at least three referees) quoting the ref. code by e-mail to elke.baum...@psi.ch or to Paul Scherrer Institut, Human Resources, ref. code 6112-04, Elke Baumann, 5232 Villigen PSI, Switzerland. More info: http://www.psi.ch/pa/offenestellen/100131 __ Meitian Wang Swiss Light Source at Paul Scherrer Institut CH-5232 Villigen PSI - http://sls.web.psi.ch Phone: +41 56 310 4175 Fax: +41 56 310 5292
Re: [ccp4bb] restraints for cis-bonds, ACD cif file
Nathaniel The atom names in the file I sent have the correct atom names as defined by the PDB for ACD. Naturally, the atom names in your PDB file and the restraints CIF file should match and given that you need the correct names to deposit your structure into the PDB, then I would recommend doing it sooner later than later. PHENIX has a number of tools to do this for you and we can talk off-line about them. Nigel On Tue, Nov 30, 2010 at 8:16 AM, Nathaniel C. Gilbert ngil...@lsu.edu wrote: I appreciate the input Paul and Nigel. The cif dictionary file you sent me doesn't have matching restraint definitions for 53 atoms in the ACD file. I opened the cif file you sent and extracted the SMILES code for ACD. Then I ran it in phenix.elbow to have it generate a pdb of the ACD. Then i would use the cif file you sent and import it into coot or phenix.refine, but it would not match the cif and pdb. I can wait for the new nightly build to come out, but I was wondering why this process wouldn't work. Paul, I'm not as familiar with the CCP4 suite. Refmac5 doesn't give me as many new Fo-Fc peaks to build more model into as does phenix.refine even though Rfree numbers are comparable. -- Nigel W. Moriarty Building 64R0246B, Physical Biosciences Division Lawrence Berkeley National Laboratory Berkeley, CA 94720-8235 Phone : 510-486-5709 Email : nwmoria...@lbl.gov Fax : 510-486-5909 Web : CCI.LBL.gov
[ccp4bb] Please unsubscribe me from CCP4 mailing list
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