[ccp4bb] Post-doctoral positions at the NKI /Amsterdam to work on Autotaxin

2011-01-19 Thread Anastassis Perrakis
Dear all, Post-doctoral positions are available in the Netherlands Cancer Institute in the group of Tassos Perrakis (http://xtal.nki.nl). Our group, together with that of Wouter Moolenaar and of Huib Ovaa, have a long-standing interest and lively collaboration in the function and

[ccp4bb] REFMAC and REMARK 3 HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

2011-01-19 Thread Huw Jenkins
I've split this off from the too many clashes thread as it's not directly related. I was under the impression that the default in refmac was to add riding hydrogens but some investigation suggests this may be only appear to be the case. If the default option in the CCP4i GUI for refmac with

Re: [ccp4bb] What is the simplest method to analytically compute the Solvent-Accessible Surface Area of a given atom in a protein?

2011-01-19 Thread Richard Edward Gillilan
Since several people have asked me for code, I now realize that I've contributed my bit to the confusion over names of various surface definitions. To clear things up there is an excellent online article which covers the history of various molecular surface definitions that I highly recommend:

Re: [ccp4bb] What is the simplest method to analytically compute the Solvent-Accessible Surface Area of a given atom in a protein?

2011-01-19 Thread Nadir T. Mrabet
It can go both ways: If you increase atom radii by adding that of the probe (e.g. 1.4 A° for a water probe) and calculate the molecular surface using a zero probe with atom radii as previously defined (Ri +1.4 A°), the area you get is that of the accessible surface. Pr. Nadir T. Mrabet

[ccp4bb] Postdoctoral Research Associate Position Available

2011-01-19 Thread Smita Mohanty
Postdoctoral Research Associate Department of Chemistry and Biochemistry Auburn University Auburn, AL 36849-5312 Project Description: The postdoctoral research associate position is available in the laboratory of Professor Smita Mohanty. The project involves structure-function studies of

[ccp4bb] PhD student position available in Norway

2011-01-19 Thread Preben Morth
Dear ccp4bb Could I get you to forward the following call to interested candidates. http://uio.easycruit.com/vacancy/498126/70932?iso=no cheers Preben J. Preben Morth, Ph.D Group Leader Membrane Transport Group Nordic EMBL Partnership Centre for Molecular Medicine Norway (NCMM) University of

[ccp4bb] increase the solubility of proteins

2011-01-19 Thread Jerry McCully
Dear ALL; Recently I am purifying a cell surface receptor by refolding of its inclusion bodies. Actually I can purify some functional monomers of this protein. However, the protein precipitates during the concentration. Interestingly, the precipitates look like crystals but not

Re: [ccp4bb] increase the solubility of proteins

2011-01-19 Thread Bosch, Juergen
If they look like crystals, why don't you shoot them and see if they diffract ? Some people have been lucky with this approach. Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North