A postdoctoral position in Cryo-EM and structure analysis is available at the
University of Texas Medical Branch at Galveston to study the structure and
active conformations of membrane-associated protein and complexes involved in
blood coagulation: http://www.utmb.edu/ncb/faculty/Stoilova-McPhi
Hello Rex,
Nice to hear from you. My contact address hasn't changed
since we communicated the last time.
I'm okay in Yokohama near Tokyo, though we are facing the
grim news and our sympathy goes to those who have suffered
in the recent quake.
Let's talk off the list.
Sincerely,
Hideaki Niwa
RIK
Dear James,
Many thanks for the detailed explanation. I do find
your results very interesting and (when time allows
!) I will certainly investigate this effect in more
detail and see if I find similar results for data
that shows significant levels of radiation damage (as
mine
Does anyone have an email address for Hide Niwa who was at Birkbeck in the
1990's and went to work back in Japan as a protein crystallographer? The
earthquake is rather worrying.
Rex Palmer
Birkbeck College
Thanks to all for your prompt, detailed and useful answers.
I thought you would all be watching the rugby this afternoon!
Rex
Dear Rex,
As Albert has already pointed out, the latest version is 7.0.7 and can be
downloaded from:
http://www.mrc-lmb.cam.ac.uk/harry/mosflm/
6.2.6 dates from about 2006 I think. It will not deal with Pilatus
detectors and there have been a very large number of improvements since
then.
For ea
According to Harry Powell's website:
The current version of Mosflm is version 7.0.7 (uploaded onto this site 22nd
December 2010).
link: http://www.mrc-lmb.cam.ac.uk/harry/mosflm
Cheers,
Albert
2011/3/13 REX PALMER
> Is MOSFILM 6.2.6 the latest version?
>
> Rex Palmer
> Birkbeck College
>
Is MOSFILM 6.2.6 the latest version?
Rex Palmer
Birkbeck College
Hi,
I'll second Israels's comment. Since the yield per coupling in synthesis is
lower for RNA than for DNA it gets really expensive over 30-35 nucleotides.
However, you can stitch together several 30 nt oligos using either T4 RNA
ligase or T4 DNA ligase (with a DNA splint).
Regarding suppliers
Hi Mike,
For long RNAs (> 40bases), in vitro transcription is the method of choice.
You might want to take a look at this introductory page from Ambion:
http://www.ambion.com/techlib/basics/transcription/index.html
For structural studies you will have to scale up to milliliter scale. For
that yo
1) Make your own with in vitro transcription (straight T7 r T7+RDRP like in
Finnzymes kit)
2) Buy from IDTDNA - they are very good
Long RNA tends to be expensive. Consider RNA ligase if two or more pieces
can be stitched together.
Artem
On Sun, Mar 13, 2011 at 3:39 AM, Michael Thompson wrote:
>
You do also have always to consider why you are doing this calculation -
usually to satisfy a sceptical and possibly ill-informed referee. A major
reason for doing this is to justify including an outer resolution shell of
data (see this BB passim), and for this I have come to prefer the random
half
The Liquidator 96 is at my opinion the best in the market. Is a manual system,
but fast without problems related to cross contamination.
The electronic ones (from other brands) at some point gives you problems with
the electroninc system and the tips are not disposable (they take long time
bet
Dear Boaz,
You are quite correct, 'latter' and 'former' need to be switched in my
email. Apologies to CCP4bb for the confusion caused!
Best wishes, George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-3
Hi Michael,
we normally produce synthetic RNAs following this classic paper if the size
is more than let say 40-50 nucleotide, otherwise we buy the RNAs from
Dharmacon and the quality is totally OK.
Hope it helps
J Mol Biol. 1995
Jun 2;249(2):398-408.
Crystallization of RNA-protein complex
Hello All,
I am looking for some advice from some experienced RNA crystallographers. I
would like to order some relatively short (<90 bases) synthetic RNAs for
crystallization trials. I was wondering if anyone could comment on the use of
synthetic RNAs for crystallization. Specifically, what is
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