Re: [ccp4bb] Trymethylamine n-oxide and PEG MME 2000

Olá Carmela, see the recent paper from Müller-Dieckmann et al. about trimethylamine n-oxide in IUCr Journal of Applied Crystallography. I think it might as well be protein crystals, is it a big effort to have them tested down the road at the ESRF? In my experience the methylated PEG makes a

Re: [ccp4bb] protein aggregation

Hi I think glycerol 10% or higher is a good choice. I have some experience with a protein that started aggregation beyond 2.5mg/ml. I tried crystallisation at this concentration with protein ligand ratio of 4:1 and was successful. On Thu, Mar 24, 2011 at 4:29 AM, vikrant saa

[ccp4bb] flex-wARP error

Hi all - I'm finding that Arp/Warp is crashing when I try to run a job. I was working on a challenging molecular replacement job where I though Arp tracing the fragmentary density could do a better job than I could by hand (this turned out to be true, by the way). I was testing different

Re: [ccp4bb] flex-wARP error

Hi - Yes, your suspicion is correct, and I think that bug has been silently fixed in the latest distribution. I am pretty sure Victor has now a job-dependent filename, if not we will try and fix it now as we prepare a new release. In that case, pleas email Gerrit telling him details

[ccp4bb] B-factor-weighted Superposition

Dear Crystallographers, do any of the commonly-used superposing algorithms weight superpositions by b-factors? Seems like it would be a good idea, so I assume it is implemented somewhere? Jacob Keller -- *** Jacob Pearson Keller Northwestern University

Re: [ccp4bb] Reminder: Fourth annual CCP4 summer school in USA, at APS, June 7-15

Dear Colleagues, This is a reminder that the deadline for applications for the 4th annual CCP4 summer school From data collection to structure refinement and beyond is April 8. The school will take place June 7-15, 2011 at the Advanced Photon Source (APS) near Chicago. There is no

[ccp4bb] Tangential Flow Filtration device

Dear all, Sorry for non-crystallography related questions. I am looking for a Tangential Flow Filtration device to concentrate large volumes of media (several liters) for protein purification. I am wondering whether I could get some suggestions on my choices. It appears that both Pall

[ccp4bb] Structures of crosslinked complexes

Dear All, Does anybody know of examples where labile protein complexes have been crystallized after using non-specific crosslinkers (so excluding complexes with engineered SS bonds, for example)? Thanks in advance for any examples, Bert On 3/23/11 10:19 AM, Parthasarathy Sampathkumar