[ccp4bb] Bypassing phase separation for nice crystals.

Hi everyone, I have been issues with a particular protein. I have been close for a while, but yet so far. Rather than going from a clear drop to crystal, my protein first undergoes phase separation (large oily drops) in which one phase contains most, if not all, of the protein. This phase

Re: [ccp4bb] Bypassing phase separation for nice crystals.

Hi Timur, have you tried seeding from your microstalline stuff? Might be worth to try! Cheers, Albert Albert GUSKOV (Dr) | Research Fellow | Division of Structural Computational Biology | Nanyang Technological University Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 GMT+8h

Re: [ccp4bb] Bypassing phase separation for nice crystals.

Original-Nachricht Betreff:Re: [ccp4bb] Bypassing phase separation for nice crystals. Datum: Mon, 18 Jul 2011 17:01:27 +0200 Von:Florian Sauer sa...@embl-hamburg.de An: F. Timur Senguen ftseng...@gmail.com CC: CCP4BB@JISCMAIL.AC.UK Dear Timur, one

Re: [ccp4bb] unusual sighting of a crystal structure

Why don't they ask us first what would be scariest? We could really come up with some good stuff JPK On Sun, Jul 17, 2011 at 2:57 PM, Eric Bennett er...@pobox.com wrote: It would be even scarier if they used an NMR structure. -Eric On Jul 16, 2011, at 1:20 PM, Robbie Joosten wrote:

Re: [ccp4bb] unusual sighting of a crystal structure

Just in case anyone want to see it IRL http://www.youtube.com/watch?v=4sYSyuuLk5ghd=1t=38s Wow! This movie is the perfect propaganda for immunology grants! JPK *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel:

Re: [ccp4bb] Off Topic: PDB validation server

On 8 Jul 2011, at 19:13, Katherine Sippel wrote: I know that the PDB updated its validation server in May as described in their news link but it seemed to indicate an increase in output options rather than a change in criteria. Is anyone aware of what changes were made to the validation

[ccp4bb] un-subscribe

Please un-subscribe me from the list! Thank you! Szilvia Szep

[ccp4bb] Data from old tapes

Dear CCP4 community, We have a few datasets that reside in tapes (Sony QG-112M 8 mm tape and Maxell DDS-2 4 mm tape). I have been searching the internet for tape drives ( and cable) but haven't found anything. Does anyone know where we can purchase compatible tape drives for these lovely

Re: [ccp4bb] Data from old tapes

My exabyte eliant 8 mm tape just went on the blink, so I've sent it to Pacific Data (http://www.pacificdata.com/tape_drive.html) for repair. They also sell tape drives, but looks like mainly newer ones. Probably have some old ones from the repair business. Search ebay for exabyte or dat tape and

[ccp4bb] off-topic: LCD monitors

Pardon the off-topic query, but I would like to get some feedback about any personal preference for 3D LCD monitors. I am trying to decide between the following 3 monitors: Samsung 2233RZ 1680 x 1050 2D and 3d Widescreen LCD Monitor Asus VG236H 23 2ms 1920x1080 Full HD 120Hz 3D multimedia

Re: [ccp4bb] off-topic: LCD monitors

If you aren't aware of this, the use of 120 Hz LCDs to display stereoscopic 3D using Nvidia 3D Vision on Apple's Operating system(s) is/are not supported: http://www.nvidia.com/object/3d-vision-pro-requirements.html However, it will work flawlessly on Linux (or Windows if you're in the mood for

[ccp4bb] Off Topic: How to delete loops from a protein

Hi all I wrote last time but got only one feedback. I know some of you guys must have this experience that how to delete loops from the protein. Please help me with suggestions. I am working with a human protein which have around 20% sequence identity with the other proteins of  the same

[ccp4bb] Off Topic: How to delete loops from a protein

Hi all I wrote last time but got only one feedback. I know some of you guys must have this experience that how to delete loops from the protein. Please help me with suggestions. I am working with a human protein which have around 20% sequence identity with the other proteins of  the same

[ccp4bb] Off Topic: How to delete loops from a protein

Hi all I wrote last time but got only one feedback. I know some of you guys must have this experience that how to delete loops from the protein. Please help me with suggestions. I am working with a human protein which have around 20% sequence identity with the other proteins of  the same

[ccp4bb] Off Topic: How to delete loops from a protein

Hi all I wrote last time but got only one feedback. I know some of you guys must have this experience that how to delete loops from the protein. Please help me with suggestions. I am working with a human protein which have around 20% sequence identity with the other proteins of  the same

Re: [ccp4bb] Off Topic: How to delete loops from a protein

You could run your sequence through this web server first: http://ffas.burnham.org/XtalPred-cgi/xtal.pl Then regarding your loops you could use MUSTANG (or any other 3D alignment tool which generates a superimposed ensemble of structures) http://www.ncbi.nlm.nih.gov/pubmed/16736488 to identify

Re: [ccp4bb] Off Topic: How to delete loops from a protein

Hi Obayed, you could give in situ protolysis a try. This is where you add a bit of protease along with you target protein to the crystallization drop. It has been quite successful for the folks at the SGC. Here are the relevant references: Dong A, et al. In situ proteolysis for protein

Re: [ccp4bb] Off Topic: How to delete loops from a protein

Hi Obayed, If I understood your question well, you are looking for something called secondary structure prediction. I googled these keywords and found this server: http://bioinf.cs.ucl.ac.uk/psipred/ You may find other interesting servers on the web and some literature comparing them. I think