Hi
you can also try dynamic light scattering of different fraction of the
peak.it will help you to know the exact molecular size of individual
peak..glutaraldehyde crosslinking is also a good option..increasing the
concentration of protein may shift the equilibrium in the direction of
higher
Hi all,
I need some advice on reproducing crystals that emerged in about 2 months from
a screen. The background is I set up a 96-well hangdrop screen and checked the
tray after 1 week and then every 2-3day for about 3 weeks. I did notice that
this particular drop seemed very dynamic overtime.
Hello Christine,
First, you want to make sure those crystals are not salt.
Crystals that appear only after a very long time are often salt
crystals that form as your drop dries out. Check diffraction to see if
it's the real thing before you invest time optimizing crystallization.
Ho
Ho
Hi Jinyi,
Do you have two versions of python installed?
Maybe executing fink remove python will solve the problem (?)
Wataru
Shoot the existing crystals. Who says you will need optmization? lol
A Postdoctoral position is available in the Cell Biology and
Biophysics Unit headed by Dr. Antonina Roll-Mecak at the National
Institute of Neurological Disorders and Stroke to study the structure
and mechanism of tubulin post-translational modification enzymes. The
Roll-Mecak Laboratory
Dear ccp4er,
I try to use Phaser MR to solve a structure. A mtz file from oasis was
used as a ensemble. All of the parameters containing protein MW, nucleic acid
MW, extent of X, Y, Z and center of X, Y, Z and RMS error were assigned.
However, when I ran phaser mr, the program complain
