Could be an organic crystal - what's the resoution of the lowest order
reflections?
Jan D.
On Fri, Oct 12, 2012 at 8:11 AM, Chang Qing robie0...@gmail.com wrote:
Hi, everyone:
I just got some strange diffraction images from crystals with
triangular pyramid shape. I think this should not be
Have you got two very similar datasets (isomorphous) inbetween which the
mentioned difference should be pronounced? You could try a cross-crystal
difference map as well.
Jan
On Fri, Oct 5, 2012 at 8:40 AM, herman.schreu...@sanofi.com wrote:
**
Dear Israel,
I wonder why you do not see
Hi,
Thanks for answering my question.
I think I'd better provide more informations. These four images were
taken from one crystals. The distance of image1-2 is 250mm, and
image3-4 is 100mm. I checked more than 10 crystals and results were
similar. The spots look very large. The lowest resolution
If you are working on a protein that binds vitamin B12 (cobalamin) then
you may be interested that there appears to be an issue with geometry of
the B12 dictionary currently distributed by ccp4. The problem is that atom
C19 in the corrin ring is defined as being SP2, planar with no hydrogen
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Dear Chang,
What makes you think spots from salt crystals should not be as large?
You have got an ordinary small molecule crystal (or probably a
cluster of them) in the beam, with a unit cell somewhat bigger than
that of ice judging by the extra
Dear All,
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Dear CCP4 community,
I have a MR solution with 2 mol/asu.
The solution looks very good in terms of packing (with reasonable starting R
and Rfree) and there are no clashes with the symmetry-mates as well.
But when I try to generate a solvent mask I get a truncated mask which covers
only 1 and
Dear Chang,
i have seen ATP diffraction, it's not very different of your image.
Maybe you have only ATP in your crystals?
Best regard
Nicolas
Le 12/10/12 14:56, Chang Qing a écrit :
Dear Tim
I think your explanation is logical. But I tried ADP as ligand first
and got crystals and
Dear Nicolas
ATP crystals is a reasonable answer. Thank you very much.
Best regard
Chang
2012/10/12 Nicolas Foos nicolas.f...@afmb.univ-mrs.fr:
Dear Chang,
i have seen ATP diffraction, it's not very different of your image. Maybe
you have only ATP in your crystals?
Best regard
Nicolas
Hi,
Sorry for off-topic question.
Does anyone have experience of the stabilisation of water-soluble proteins
by detergents? Protein I'm working with is definitely water-soluble and has
high yield, but, unfortunately, not very stable. Especially during
concentration. So, we thought that adding
Hi all,
Does anyone a program/paper that would give some quantitative estimate for
protein solubility based on surface property analysis?
(excluding obvious things such as integral membrane / TM regions)
Best
Tommi
Hi,
Yes, it is worth trying. Nonionic detergents can be good for this.
One example I know of and readily comes to mind is the use of 0.1% NP40
(Noniondet P40) in the stabilization of murine reverse transcriptase during
purification (also helps toprevent precipitation), first described in:
Hi,
Using a surface property analysis (3D structure is available), I think you can
get a quantitative estimate by using PISA to find the solvent-accessible area
and salvation energy and then comparing that to corresponding values obtained
by using in your lab another protein for calibration
The following paper (which can be found at
www.wolfson.huji.ac.il/purification/PDF/Literature/Bondos2003.pdf
Detection and prevention of protein aggregation before, during, and after
purification. Sarah E. Bondos and Alicia Bicknell (2003) Analytical
Biochemistry, 316, 223-231
contains a
Hi, Thanks, i was thinking of a situation where one can model the sequence to a
structure based
on homologous structures, ie assuming i know the fold - i guess PISA / ASA
based estimates
are the first thing.
(mainly indeed the likelyhood to aggregate ie the surface property estimates
are
Vitali,
Echoing what Dan said, I am not sure why you have chosen detergents first, as
there are many other agents which stabilize proteins. Is the instability due
to hydrophobic surfaces (e.g., made worse at higher salt) or not. Some
non-detergent suggestions are:
1) diols like MPD (see
On Mon, Oct 08, 2012 at 06:20:59PM +, Ronan Keegan wrote:
Dear CCP4 Users,
A CCP4 update has just been released, consisting of the following changes:
Hi Ronan et al,
The update client on OS X doesn't seem to like our installation and dies
with:
Can't make class cfol of alias
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