[ccp4bb] off topic question about amount measurements of Membrane protein

2013-09-30 Thread Yu, Kun
Dear all, I am a new starter in the membrane protein field and working with the cell free expression system. As the really small amount of protein I could get in hand, I got somehow stuck in the quantification of the protein yield. so Is there any suggestion of any methods or reagents suitable

Re: [ccp4bb] Is there someone work with Lipids?

2013-09-30 Thread Ed Pozharski
May I ask a question? This is a catch-22 situation. If you ask permission to ask a question, you are already asking a question. Answering this with no would probably create a wormhole. I'd also like to see a bulletin board where asking questions requires separate permission. When we