Hi,
If there is more than one strong unique Patterson peak, then Phaser currently
doesn't know how to interpret the tNCS. If all the peaks can be interpreted as
multiples of one one vector (taking symmetry into account) then you can use the
NMOL argument of the tNCS command. Otherwise, you can
Hi,
Having now had a chance (finally) to play with the Asus VG27A, I can
say that (a) it works really well in stereo with both Coot and PyMol and (b) it
is easier and nicer to use than the Zalmans.
Though the technology is essentially the same (halving the vertical
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Hi,
(and apologies if ANYONE has seen this twice - CCP4BB rejected my first
post as coming from an unknown (i.e. Leeds) address, and the second one because
I had already posted from my Leeds address….)
Here goes:
Having now had a chance (finally) to play with the Asus
Dear Frank,
It is difficult to say something without the diffraction images but probably
they are detergent ( or lipid) crystals. These crystals are in general soft
and don't diffract well.
My suggestion is to know well what your crystals really are before any
optimization.
Best,
Isabel
The problem you're having is a very common one, unfortunately. There are about
10,000 things you can try to improve diffractiongenerally going from low to
solvable resolution is very, very hard.
I would say the two most important ones are (i) to vary the detergent and (ii)
to go to another
Hi Frank,
unfortunately it is very common with membrane protein crystals to get stuck
with diffraction quality around 20A.
From what you describe you could consider the following:
a) revise the detergent you solubilize your MP with (e.g. use OG instead of
DDM), and consider to change to
Hi Frank,
Do not forget that membrane protein crystals are often fragile and
difficult to manipulate.
Finding good cryo condition can be difficult and small temperature
variation can destroy crystals
within minutes, this makes room temperature diffraction tests not always
obvious. The time
Hi David
Nothing to do with the iMosflm developers. CCP4 has made some changes
that have caused this error to arise in 6.4.0 on Linux (this is not
to say that the iMosflm code didn't have the bug, just that it
doesn't appear with either our own version or with the same version
Hi Frank,
The previous suggestions are great. In my case, I had soft crystals (bend from
180° to ~130-140°) in the loop using the LCP but they still diffracted. There
are a lot of heroic stories on how some people solved a structure, you should
just try as many ways as possible (do not only
Dear Frank,
Toufic is right. The trick is no trick, you just have to try everything. In
my experience if u have a protein crystals in a mild detergent and it is
very fragile no point going to a harsh or shorter chain detergent like OG.
I got crystals in NM (9 C) and they were fragile and dissolved
Hi everyone,
Sorry for being a bit off topic, but I thought this group would be great for
advice.
I’m looking at upgrading my current laptop to a newer MacBook Pro. I’m torn as
to whether I need integrated vs discrete graphics for structural biology,
including molecular modelling, especially
Hi,
Can I profit from Kristin's question and add one: is it too soon to know if the
crystallography software (CCP4, Coot, Autobuster, XDS) will work fine with
Mavericks (Mac OS X 10.9)?
(I remember a bit of trouble when Lion came off).
Carlos
Em 23 oct. 2013, às 22:10, Kristin Low
I'm currently using CCP4/COOT (from the official installer) and autoPROC on
Mavericks without any problems. I imagine the rest of the global phasing tools
will work nicely.
I had issues with fink, you have to use a branch from github as well as
manually install and update the Command Line
Hi David,
I've just renamed the ipmosflm in $CCP4/bin to ipmosflm_new and then
defined
MOSFLM_EXEC as /path/to/ccp4-6.3.0/bin/ipmosflm.
CCP4 6.3 needs to be installed for this to work.
Andreas
On 23/10/2013 5:39, David Schuller wrote:
iMosflm developers:
I have installed CCP4 6.4.0,
On Wed, Oct 23, 2013 at 1:10 PM, Kristin Low kristin@queensu.ca wrote:
I’m looking at upgrading my current laptop to a newer MacBook Pro. I’m
torn as to whether I need integrated vs discrete graphics for structural
biology, including molecular modelling, especially since the latest
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