Hi Zhijie,
Thank you so much for the reply. The protein I am working on is from eukaryotic
cytosol. We've already tried optimize the IPTG concentration and induction
temp. Co-expression of chaperons seems to be a interesting idea, I googled and
found this one:
http://www.clontech.com/takara/US
Dear Colleagues,
I would like to make you aware of the following opportunity for final year
undergraduate and Masters-level students. The University of Birmingham and GSK
have initiated Coopera-TB, an EU-funded consortium focussing on hit to lead
optimisation of novel anti-TB scaffolds.
Detai
*Dear Eleanor,
There is a reason why I do not provide a routine to go from the .phs
from shelxe to .mtz. If he uses the 'free lunch' option
in shelxe he may finish up refining against the free lunch structure
factors. According to one user who managed to do this
by accident, this gives excellent
It is easy enough if you still ant to do it.
I would feed both into f2mtz separately to make a mysadIplus.mtz and a
mysadImin.mtz
then use CAD to combine and change the default labels to something like I(+)
SIGI(+) and I(-) SIGI(-)
But as George says Why do you want it? That will change the way