Thank you guys for the replies! I tried the PISCO method from Marlene Holder.
It is pretty cool, and clear method which could used directly in PYMOL. Thank
Thomas Holder for the help of PISCO installation!
I also tried the method from Matthew Franklin. From this method, I learned the
theory
If cost is an issue, I recommend preparing your own MINI Dialysis
Devices at 99.74% savings.
Cut a hole in the lid of an Eppendorf tube, put your sample in the tube,
then close the lid over a small piece of dialysis tubing, trapping it as
a cover. Float inverted in dialysis buffer and Bob's
For even smaller volumes, make a dialysis button from an eppendorf tube:
With a razor blade, cut the tube off leaving only the rim
that the cap snaps into.
Put your sample in the depression in the middle of the cap (inside).
cover with membrane and snap the rim down over it.
(I have done this but
