Re: [ccp4bb] In search of an article

2014-11-03 Thread Uwe Sauer
Hi Philippe, for the version with nylon fibres have a look at: Sauer and Ceska (1997) A simple method for making reproducible fibre loops for protein cryocrystallography, J. Appl. Cryst. 30, 71-72 regards, /Uwe On 2014-11-03 18:23, DUMAS Philippe (VIE) wrote: I am looking for the reference

[ccp4bb] Postdoctoral Fellow Position – Structural Biology at Rutgers University School of Dental Medicine

2014-11-03 Thread Vijay Parashar
A postdoctoral position is available immediately in the Department of Oral Biology Rutgers School of Dental Medicine for a highly motivated, creative individual with strong interest in the structure and function of proteins regulating bacterial signaling. The ideal candidate will have a strong

Re: [ccp4bb] In search of an article

2014-11-03 Thread DUMAS Philippe (VIE)
Le Lundi 3 Novembre 2014 19:06 CET, "Frances C. Bernstein" a écrit: Thank you all ! I got it. Philippe D > How appropriate that someone whose name is Picot would be > the first one to have the answer. > > If you don't get it, put define:picot in a google search box. > >

Re: [ccp4bb] In search of an article

2014-11-03 Thread Frances C. Bernstein
How appropriate that someone whose name is Picot would be the first one to have the answer. If you don't get it, put define:picot in a google search box. Frances = Bernstein + Sons * * Informat

Re: [ccp4bb] In search of an article

2014-11-03 Thread Daniel Picot
Hi Phhilippe, T. Y. Teng, J. Appl. Crystallogr. 23, J. Appl. Crystallography 23:387–391 (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film A method for mounting single crystals in macromolecular crystallographic studies is described in which the crysta

[ccp4bb] In search of an article

2014-11-03 Thread DUMAS Philippe (VIE)
I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to make a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas

Re: [ccp4bb] fastening crystal formation

2014-11-03 Thread Appu kumar
Hello, If the homologous protein structure is available, or you have symmetry information of your crystal, then try engineering surface by putting in residues which has less entropy like replacing Lys to Arg, Met to Gln. We are able to fasten the crystal growth from 20 days to 3-4 days by creating

Re: [ccp4bb] Your top choice of crystal screens!

2014-11-03 Thread Morten Grøftehauge
Fabrice Gorrec, at Cambridge, made the MORPHEUS screen and I really, really like it http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246824/ It's easy to mix yourself and if you make extra of the stocks it is also very easy to mix a follow-up grid screen. The conditions are cryoprotected from the outse