[ccp4bb] Senior Research Assistant Position - Protein purification, cloning and biophysical binding studies

Dear all, This post is to bring your attention to a job opportunity for a Senior Research Assistant position at the University of Texas, MD Anderson Cancer Center (in Houston, Texas, USA). MD Anderson Cancer Center is the number one cancer center in the United States and a center of excellence

Re: [ccp4bb] Large scale mammalian expression system recommendation?

Hello All, I would second the notion that transient systems are quite good for testing expression levels on the small scale and can be used for scale up (to a certain degree at least). It is probably the fastest way to go to screen a number of constructs. Cheers, tom -Original Message--

[ccp4bb] Postdoctoral Research Assistant in Biomolecular Simulation and Protein Design

Dear all, Applications are invited for a Postdoctoral Research Assistant to work in the research group of Dr. Alessandro Pandini at Brunel University London. This appointment is funded by the Leverhulme Trust for 24 months. The research project is in collaboration with Dr James Garnett at Queen

Re: [ccp4bb] Large scale mammalian expression system recommendation?

Hi Nate, There can be no consensus I guess, most importantly because transmembrane proteins are so diverse :-) We typically use transient transfection at the screening stage, and often for large-scale expression (e.g. PMID 24909990). Bac-Mam has some advantages (nicely described in 25299155, 2704