[ccp4bb] Native Patterson Plot

Hi Fellows/Developers, I am trying to create a native Patterson map plot, but in ccp4i on windows the PS plot file (*.plt) is mutilated https://www.dropbox.com/s/f3cl2980qzws3gj/mono_15_1.plt?dl=0 (wrong orientation & partly off paper) when viewed in ghostview. I vaguely recall these being

Re: [ccp4bb] Open SESAME & Instruct-ERIC Workshop on Remote X-ray Data Collection from European Synchrotrons at the Weizmann Institute of Science on 14-18 May 2018

Dear Rik and Joel, This is really great. Thank you very much. I will follow it from New York. Have a great workshop. regards, Rajesh ---x With regards Rajesh K. Harijan, Ph.D. Schramm Laboratory Albert Einstein College of Medicine 1300 Morris Park Ave., Bronx, NY 10461 Tel:

Re: [ccp4bb] Open SESAME & Instruct-ERIC Workshop on Remote X-ray Data Collection from European Synchrotrons at the Weizmann Institute of Science on 14-18 May 2018

I will check again with her tomorrow early morning Sent from my iPhone On 8 May 2018, at 22:52, Rikkert Wierenga > wrote: Dear All, There will be an Open SESAME & Instruct-ERIC Workshop on Remote X-ray Data Collection from European

[ccp4bb] Open SESAME & Instruct-ERIC Workshop on Remote X-ray Data Collection from European Synchrotrons at the Weizmann Institute of Science on 14-18 May 2018

Dear All, There will be an Open SESAME & Instruct-ERIC Workshop on Remote X-ray Data Collection from European Synchrotrons at the Weizmann Institute of Science on 14-18 May 2018. For those who are not able to come, the entire workshop will be streamed online, password not required, starting

[ccp4bb] Postdoctoral position: Structural and chemical biology of DNA repair nucleases

We have an exciting opportunity for a Postdoctoral Research Scientist at the Structural Genomics Consortium in Oxfrod. You will join a collaborative project supported by Cancer Research UK; you will perform structural analysis of the MBL proteins (SNM1A, SNM1B/apollo and SNM1C/artemis), in

Re: [ccp4bb] [ccp4bb] tNCS problem

Hi, Phaser is pretty robust now if you have 2-fold tNCS (indicated by having only one large non-origin peak in the native Patterson map), and recent versions are better at dealing with higher order tNCS (i.e. 3 or more copies related by multiples of a single translation vector). However,

[ccp4bb] EM Research Associate job opening at Harvard Medical School

*Research Associate position at Harvard Medical School* Previous EM experience is helpful but NOT required. A position of EM Research Associate (with lab managing responsibility) is immediately available in the Laboratory of Maofu Liao (https://liao.hms.harvard.edu/) at Harvard Medical School in

Re: [ccp4bb] AW: Re: AW: [ccp4bb] tNCS problem

On a side note, I assume that you have searched with both P2 as well as P21 for your space group. If not, I will point out that there are 265 submissions in the rcsb for the P2 space group, compared to 20,698 for P21. That makes P2 fairly rare. Good Luck! - Todd

[ccp4bb] AW: Re: AW: [ccp4bb] tNCS problem

Dear JiYG, With an unknown number of 1000 aa monomers, you definitively have a challenging project! In that case, you will have to do some homework before running phaser: 1) The Matthews program should give you an indication how many monomers to expect. 2) P2 is a very low symmetry

Re: [ccp4bb] AW: [ccp4bb] tNCS problem

Dear JiYG, Just to mention also that it was impressed on me in a similar situation, either in my reading a ccp4bb thread or Randy directly, that the new version of Phaser (early 2017 onwards) was improved for such situations. Since you will no doubt keep your Phaser up to date anyway you should

Re: [ccp4bb] AW: [ccp4bb] tNCS problem

Dear Herman, I tried phaser. It worked. But there are a lot of crashes in the structure. Meanwhile, R factors could not be lowered. I tried to use P1 to process the data, but I do not know how many monomers in the asymetric unit. Bests, JiYG  On 05/08/2018 22:50,

[ccp4bb] AW: [ccp4bb] tNCS problem

Dear JiYG, Unless the tNCS has caused processing problems, Phaser should automatically deal with tNCS and I would recommend to just give it a try. If it fails, you could try more sophisticated approaches. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von ???

[ccp4bb] tNCS problem

Dear all, Recently, I collected a data set of a crystal of a big protein, which contains 1000 amino acids. I processed the data to P2. But the data set has tNCS problem. I want to do molecular replacement. Is there anyone know how to deal with this problem?

[ccp4bb] Open PhD Position at MPI Tübingen, Germany

Dear all, we are offering a * PhD position at the intersection of medicinal chemistry and structural biology * at the Max Planck Institute for Developmental Biology in Tübingen, Germany. We currently offer a project on the development of a novel anti-pathogenic drug for the prevention

Re: [ccp4bb] Introducing Dials User Interface for CCP4

It also runs for us! Thanks _ Dr. Martin Martinez-Ripoll Research Professor Emeritus xmar...@iqfr.csic.es Department of Crystallography & Structural Biology www.xtal.iqfr.csic.es www.xtal.iqfr.csic.es/Cristalografia/ Telf.: +34 917459550 Consejo Superior

[ccp4bb] MoRDa update

Dear All MoRDa is a pipeline for molecular replacement structure solution. A new update to the package is now available. The structure solution program is improved and database is extended . Both update and installation instructions are available from Morda homepage