Re: [ccp4bb] Assumptions on protein activity (again)

Hello Markus, As has been mentioned by others, there are many ways to inactivate or inhibit a protein's activity. Why do you assume your protein is unfolded? From the information you have given, the protein is folded, just inactive or has low activity under several buffer conditions other tha

Re: [ccp4bb] Assumptions on protein activity (again)

Dear Markus, it is not trivial to find the reason why your protein activy is sensitive to the purification procedure. Assume your active site contains a cysteine (e.g. a cysteine protease) and only one of your four conditions contains reducing agent during the purification. In the other three

[ccp4bb] Assumptions on protein activity (again)

Dear all Thanks to for all the responses. I would continue my question for getting more advice. I expressed a dimeric multi-protein (600 KDa). This protein was purified (affinity chromatography) in 4 different common buffers and run with the same SEC-buffer. We observe clear single size-exclusion

Re: [ccp4bb] Looking for alternatives for discontinued crystallography items

Hello, You may check CombiPuck kit Mitegen website https://www.mitegen.com/?s=combipuck+kit https://www.mitegen.com/product/combipuck-system/ which may hold 16 cryo vials with crystal pins. Th

[ccp4bb] Travel bursaries for CCP4 Study Weekend 2019 - deadline for applications 31st October 2018

Travel bursaries are available for students and young Post-docs from non-UK labs to come to CCP4 Study Weekend 2019. Deadline for applications is Wednesday 31st October 2018. For more info see http://www.cvent.com/d/sgq8q6/1K?cpc=N7N8SK89V8N Regards Karen McIntyre Scientific Computing Departme

[ccp4bb] Standard student bursaries to CCP4 Study Weekend 2018

Standard student bursaries are available to all students registering for the CCP4 Study Weekend 2019 in the early bird period (ends 18 November 2018). This covers the cost of registration and one night's accommodation in halls. Regards Karen McIntyre Scientific Computing Department - CCP4 R92 1

Re: [ccp4bb] Looking for alternatives for discontinued crystallography items

Hi Matthew In the UK Molecular Dimensions sell the cane sleeves – since you can get the prices in dollars I’m sure they ship internationally https://www.moleculardimensions.com/products/3808-CryoSleeve/ I’m not aware of the cryo vial holders but Molecular Dimensions still sell (well they are l