[ccp4bb] SEC and MALS

2019-08-26 Thread Natesh Ramanathan
Dear Friends, Can you share your experience with examples of MALS giving lower molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg. Dimer), for the same protein sample? If you have/know any published paper, can you please point me to that reference paper or

[ccp4bb] RFP for Drug Discovery - In Silico Small Molecule Screening Awards

2019-08-26 Thread Nick DeHaan
Hi CCP4BB Community, I’m excited to announce that the next round of the Atomwise AIMS Awards program is now accepting proposals! The award program provides in silico small molecule screening as well as compounds and is open to all non-profit research institutions, including international

Re: [ccp4bb] SeMet data

2019-08-26 Thread Nukri Sanishvili
Hi Lindsey, Obviously, one would need a lot more information to properly diagnose the problem and I am sure much smarter people them me will ask you for that. But just to move the task by couple of steps, I want to point out couple of things. 1. Trivial question: did you have the anomalous option

[ccp4bb] Postdoc position in San Antonio, Texas

2019-08-26 Thread Yogesh Gupta
*Postdoctoral Fellow position at UT Health San Antonio, San Antonio, Texas* The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at University of Texas Health Science Center in San Antonio (UT Health San Antonio™) is seeking a motivated scientist with strong interest in

Re: [ccp4bb] SeMet data

2019-08-26 Thread Keller, Jacob
Why do you think you are rejecting anomalous data? What do the normal tell-tales reveal, like anom CC? JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk: (571)209-4000

[ccp4bb] SeMet data

2019-08-26 Thread L. Doyle
I have some Seleno-Methionine protein crystals (12 SeMet of 211 amino acids, incorporation verified by Mass Spec). I've already collected several datasets (ALS BL5.0.2) but I seem to be losing (rejecting?) a lot of anomalous signal during data processing. I'm most familiar with HKL2000, but I

Re: [ccp4bb] Fo-Fc map in WinCoot

2019-08-26 Thread Raymond Brown
Yes that works like magic. Never noticed that little box before. WinCoot is jsu wonderful. Hours of endless fun awaits me. Many thanks Ray Brown On Monday, August 26, 2019, 10:47:51 AM EDT, Jonathan Cooper <0c2488af9525-dmarc-requ...@jiscmail.ac.uk> wrote: When you open the map,

Re: [ccp4bb] Fo-Fc map in WinCoot

2019-08-26 Thread Jonathan Cooper
When you open the map, in the window which comes up there is a little box to tick which says "Is difference map?" in the lower left hand corner from memory. Then it will display the -ve contours. Sent from Yahoo Mail on Android On Mon, 26 Aug 2019 at 15:34, Raymond Brown wrote: Hi

[ccp4bb] Fo-Fc map in WinCoot

2019-08-26 Thread Raymond Brown
Hi folks, I notice that WinCoot does not appear to display the negative peaks in Fo-Fc difference maps. Is there a fix for this? Best Ray Brown To unsubscribe from the CCP4BB list, click the following link: