Re: [ccp4bb] Unusual monomer-monomer interface in crystal

2020-01-31 Thread Ivan Shabalin
Chris, Let me add my five cents to the discussion. Processing your data to keep I+ and I- merged separately is a very good practice, as noted by Eleanor and Mitch. Also, I highly recommend using the CMM server (https://csgid.org/csgid/metal_sites/) to validate the correctness of the metal.

Re: [ccp4bb] Mixed oligomeric states in crystallo

2020-01-31 Thread Diana Tomchick
Here’s two examples of heterooligomers that crystallized in a lattice with an extra monomer of one of the proteins. In both cases this was an unexpected result, but easily explained due to the low micro molar affinities for the complex. 4PKY 2CJS Diana

[ccp4bb] Fwd: Open scientist position at the EMBL-Grenoble - Project leader for ID30A/MASSIF-1 beamline upgrade

2020-01-31 Thread Andrew MC CARTHY
Dear all, Just a quick reminder that the closing date for applications is in one week (Feb 7th). We have an open scientist position at the EMBL-Grenoble for a Project leader to oversee our contribution to the upgrade of the fully automated ID30A/MASSIF-1 macromolecular crystallography

Re: [ccp4bb] Mixed oligomeric states in crystallo

2020-01-31 Thread orly avraham
Hi, To my knowledge, the structure of the rhizavidin-biotin complex contains a hexamer and a monomer, where the monomer forms a dimer using symmetry operations. PDB id: 3ew2. Best, Orly On Fri, Jan 31, 2020, 17:33 Kluenemann, Thomas < thomas.kluenem...@helmholtz-hzi.de> wrote: > Dear all, > >

[ccp4bb] AW: Mixed oligomeric states in crystallo

2020-01-31 Thread Schreuder, Herman /DE
Dear Thomas, The asymmetric unit of the bovine pTAFI structure (3dgv) contains one regular dimer and one somewhat disordered monomer. However there is no domain-swapping of alpha-helices or other special things. Best regards, Herman Von: CCP4 bulletin board Im Auftrag von Kluenemann,

[ccp4bb] postdoc position

2020-01-31 Thread Alexandra Deaconescu
A postdoc position is available in my lab. Please see below for more information. Thank you. ** * * *POSTDOCTORAL POSITION* *at Brown University, USA* The Deaconescu Lab at Brown University has one opening for a postdoctoral researcher. The laboratory's interests lie primarily in stress

[ccp4bb] Mixed oligomeric states in crystallo

2020-01-31 Thread Kluenemann, Thomas
Dear all, We recently solved a the structure of a small c-type cytochrome. We observed, that of the eleven chains in the asymmetric unit ten form 3D domain swapped dimers by exchanging an α-helix. The eleventh chain is present as a monomer. Based on the anomalous iron signal and the chain

[ccp4bb] Electron microscopy support position @ CSSB, Hamburg

2020-01-31 Thread Thomas White
Hi everyone, The CryoEM facility at CSSB in Hamburg is growing (https://www.cssb-hamburg.de/facilities/cryo_em/index_eng.html) We are currently looking for an EM support scientist to complement our Team. A job description is pasted below - however, we are totally aware, that everyone

[ccp4bb] CryoEM and beyond - an exciting postdoc opportunity in Exeter

2020-01-31 Thread Daum, Bertram
Dear all, An exciting and highly interdisciplinary ERC-funded postdoc position is now available in our lab at the Living Systems Institute in Exeter, UK. https://www.exeter.ac.uk/livingsystems/ We are looking for an enthusiastic researcher to investigate the structure and function of S-layers