Dear Thomas, The asymmetric unit of the bovine pTAFI structure (3dgv) contains one regular dimer and one somewhat disordered monomer. However there is no domain-swapping of alpha-helices or other special things.
Best regards, Herman Von: CCP4 bulletin board <[email protected]> Im Auftrag von Kluenemann, Thomas Gesendet: Freitag, 31. Januar 2020 16:23 An: [email protected] Betreff: [EXTERNAL] [ccp4bb] Mixed oligomeric states in crystallo EXTERNAL : Real sender is [email protected]<mailto:[email protected]> Dear all, We recently solved a the structure of a small c-type cytochrome. We observed, that of the eleven chains in the asymmetric unit ten form 3D domain swapped dimers by exchanging an α-helix. The eleventh chain is present as a monomer. Based on the anomalous iron signal and the chain tracing we are sure that no chain was missed. I tried to find other examples in the PDB, were one crystal is made of different homo- or heterooligomers. I only found proteins with partial occupied peptide binding sites, which is not what I am looking for. Does anyone know of a case were the presence of different homo- or heterooligomers is required to form the crystal? Best regards, Thomas Klünemann ________________________________ Helmholtz-Zentrum für Infektionsforschung GmbH | Inhoffenstraße 7 | 38124 Braunschweig | www.helmholtz-hzi.de<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.helmholtz-2Dhzi.de&d=DwQGaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=DeQlQpahGfl-vy0vrH4-_BGAMKCCEpFz2UJh9KBc0WU&s=gjglCkYgZUh6caaBeP00bN3Phiz7Bbt11cjmQ40Ct-M&e=> Vorsitzende des Aufsichtsrates: Frau MinDir'in Prof. Dr. Veronika von Messling Stellvertreter: MinDirig Rüdiger Eichel, Niedersächsisches Ministerium für Wissenschaft und Kultur Geschäftsführung: Prof. Dr. Dirk Heinz; Silke Tannapfel Gesellschaft mit beschränkter Haftung (GmbH) Sitz der Gesellschaft: Braunschweig Handelsregister: Amtsgericht Braunschweig, HRB 477 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMGaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=DeQlQpahGfl-vy0vrH4-_BGAMKCCEpFz2UJh9KBc0WU&s=wDzG_JJBUhTkvULznMip8kpyvzH9iLsVt9_qIuayeyk&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
