Re: [ccp4bb] MX data processing with GPUs??

Dear Ana, To benefit from GPU architecture, over CPU, the algorithm needs to do quite significant number crunching – i.e. do at least certain number of floating point operations (FLOP) per one byte of data. It also needs to be highly parallel, preferably without conditional (if/else) statements

Re: [ccp4bb] [3dem] Which resolution?

Hi Pawel, We can indeed agree to disagree upon many basic things in life. We apparently disagree on the basic assumptions upon which you choose to build your science! What I am criticising is the very foundation you use to construct your science, namely the flawed Frank & Al-Ali (1975) formula r

Re: [ccp4bb] MX data processing with GPUs??

Dear Ana, how do you deal with the crescent amount of data we are facing, at Synchrotrons and XFELs? At LCLS, we plan to stream data to near-by supercomputing facilities for serial crystallography data processing. So we are developing code to fully utilize both CPUs and GPUs on these machines.

Re: [ccp4bb] MX data processing with GPUs??

Dear Ana, you may want to check how many single board computers (like the Raspberry Pi or true open source hardware platform) you can buy for a single GPU high end graphics card. It may be cheaper at better performance compared to GPU computing. XDS (and possibly also DIALS) can distribute its

[ccp4bb] MX data processing with GPUs??

Dear all we have asked this of a few people, but the question remains: does any of you have experienced/tried using GPU based software to treat MX data? for reducing or subsequent image analysis? is it a lost battle? how do you deal with the crescent amount of data we are facing, at Synchrotrons

[ccp4bb] Early bird registration extended - CCP-EM Spring Symposium VI - 27-29th April 2020

Dear all, Please note we have extended the early bird registration period for the CCP-EM Spring Symposium untill this Friday, 21st February. After that the registration fee will increase! Full details are available here: http://www.cvent.com/d/8hqsdb CCP-EM are proud to announce the sixth an

Re: [ccp4bb] FW: [ccp4bb] [3dem] Which resolution?

Dear John, I really like your lecture notes! My Imperial/Leiden lecture notes – currently being updated – look a lot like yours. The earlier version: (https://www.single-particles.org/methodology/MvH_Phase_Contrast.pdf) And you are fully correct: “resolution of cryoEM imaging varies locally”! T

Re: [ccp4bb] disulfides in coot

Hi Sam, Once a disulfide bridge is made Coot will restrain the sulfur atoms to bind. The way out is deleting one of the side chains and adding it back while making sure the sulfurs do not get too close. HTH, Robbie On 18 Feb 2020 11:24, Sam Tang wrote: Dear all A very technical question whic

[ccp4bb] disulfides in coot

Dear all A very technical question which I believe a few simple mouse clicks would solve. Is there a way I can ask Coot not to build the disulfide linkage automatically (which lies within a strong red density)? My WinCoot is version 0.8.9.2 Many thanks! Regards Sam ###

[ccp4bb] Cryo-EM sample preparation workshop at the ESRF / 9th to 11th of June 2020

Dear All, We are pleased to announce our 2.5-day practical workshop on sample preparation for single particle cryo-EM from *June**9^th to 11^th2020*. This is the fourth of a series of practical hands-on workshops and is aimed at PhDs, PostDocs and scientists new to the field of single particl