Hi Aaron, thank you for your tribute to an extraordinary scientist; It brought
back happy memories.
Jack Dunitz visited Australia in the late ‘80s, possibly for the IUCr meeting
in Perth, and I can recall his razor wit and much hilarity when he, Sandy
Mathieson, and Jim Morrison were
Dear CCP4 community,
I would like to draw your attention to the Macromolecular Xtallography
Raw Data Repository (MX-RDR). The repository, supported by EU funded
Operational Programme Digital Poland, was created by the
Interdisciplinary Center for Mathematical and Computational Modelling of
hey,
my information is that detecting phosphorylation via ms is non-trivial because
the P groups are lost during fragmentation and prior to detection.
cheers
jon
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board Im Auftrag von Wim Burmeister
Gesendet: Mittwoch, 15. September 2021
Phos-tag gels are an option (albeit a fiddly one).
Cheers,
Pat Loll
___
Patrick J. Loll, PhD (he, him, his)
Professor of Biochemistry & Molecular Biology
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St.
Philadelphia, PA 19102-1192
Hello,
try mass spectrometry, either after tryptic digestion into peptides or
electrospray MS on the entire protein if it is not too big.
Best
Wim
- Mail original -
De: "Andrea Moretti"
À: "CCP4BB"
Envoyé: Mercredi 15 Septembre 2021 14:28:45
Objet: [ccp4bb] Phosphatase enzymatic assay
Dear CCP4 community,
sorry for the off topic question but I am struggling with enzymatic
assays and I would need your help.
I am trying to measure dephosphorylation of a phosphorylated kinase by a
ser/thr phosphatase. So far I tried to measure it by malachite green
assay, anti pSer/pThr
We are glad to announce the call for synchrotron beamtime applications
in biological small-angle X-ray scattering (SAXS) and macromolecular
crystallography (MX) for the period February - December 2022. The
following EMBL beamlines at PETRA III are available: P12 (SAXS), P13 and
P14 including
Dear Manfred,
thanks a lot. I had checked the homepage before. There is a note that
the software is not any more available for download from their site.
But no further hint whether it is available somewhere else.
Best regards,
Guenter
https://agklebe.pharmazie.uni-marburg.de/
Cheers,
