Dear AllThank you for your infornation regarding this vectorBest RegardsRana
Dear CCP4Sorry for the question, does anyone work with the pMAL-p2X vector not
c2X, because I am trying to find this vector for a long time now, and neither
NEB of life technologies provide this vector. Does anyone know from where I
can get it or any company provides it, I would be grateful.
Dear All
Thank you for your suggestions and articles regarding the cryo-EM
Best Regards
Rana
Dear CCP4
I wanted to ask has anyone tested 3D protein structure by cryo-electron
microscopy? what is the suitable concentration required for this procedure
Best Regards
Rana
Dear CCP4
Does anyone know how to remove the maltose binding
protein after cleavage from the target protein; I have tried gel filtration and
ion exchange but with no luck, my protein is interacting with the MBP even
after complete cleavage. I would be grateful for any help or suggestions
Best
, is there anything that could
maybe precipitate the MBP
Best Regards
Rana
From: Mark J van Raaij mjvanra...@cnb.csic.es
To: rana ibd rna19792...@yahoo.com
Sent: Thursday, March 27, 2014 11:35 AM
Subject: Re: [ccp4bb] maltose binding protein
did you try
Dear CCP4
Thank you for all your suggestions
Best Regards
Rana
Dear CCP4
Does anyone know a good cryoprotection condition for these two conditions I
would be grateful
The first condition:
10% PEG 2, 20%PEG MME 550. 0.03M of NPS ( NPS is sodium nitrate, disodium
hydrogen phosphate, and ammonium sulfate), 0.1MMES/Imadizole pH 6.5
The second condition:
Dear ccp4
I
wanted to ask if anyone has expressed DDB1 (Damaged DNA Binding protein) or
any other interacting partner using
bacterial cells instead of insect or mammalian or yeast cells, I also wanted to
ask if
anyone has any experience with this binding partner interacting with a fusion
Dear Debasish
What do you mean by percentage? do you mean consentration? so if you mean cons.
I think you should test you protein using a TCP kit to observe at what cons.
would your protein precipitate, this way you would verify the convinient cons.
for your protein before crystallization
Dear CCP4
I am having a problem with cleaving my fusion protein and I would be
grateful if you advice me regarding this situation, I have an MBP-DHBx fusion
protein and I am trying to cleave it using TEV protease, I have tried different
ratios and different temperatures with different
Dear All
Thank you for all your replies, the buffer for the TEV protease that I have
used contains 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, and 1mM DTT at PH= 8.0. I
have tried using this buffer without NaCl but the TEV protease precipitates
when dialyzing over night, as for using glutathione
Dear CCp4
Thank you for all your and information suggestions regarding question
Best Regards
Rana
Dear ccp4
I am working on hbx (Hepatitis B x protein for both that duck and the human)
I worked first on the dhbx which contained the vector pRSET-A and I didn’t get
any results, then I changed the vector ( thanks to many of you who provided me
with information, papers and books) using
Dear ccp4
I am working on hbx (Hepatitis B x protein for both that duck and the human)
I worked first on the dhbx which contained the vector pRSET-A and I didn’t get
any results, then I changed the vector ( thanks to many of you who provided me
with information, papers and books) using
Dear all
yes my paalet is large enough and no my protein is not soluble ,and I am using
37degrees before and after induction with IPTG,and the vector that I am using
is pRSETA 3.2kd
Best Regards
Rana
From: Kelly Daughtry kellydaugh...@gmail.com
To: rana ibd
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