Dear All 
   Thank you for all your replies, the buffer for the TEV protease that I have 
used contains 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, and 1mM DTT at PH= 8.0. I 
have tried using this buffer without NaCl but the TEV protease precipitates 
when dialyzing over night, as for using glutathione (reduced alone, also with 
the oxidized) still had the same result

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