Dear All Thank you for all your replies, the buffer for the TEV protease that I have used contains 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, and 1mM DTT at PH= 8.0. I have tried using this buffer without NaCl but the TEV protease precipitates when dialyzing over night, as for using glutathione (reduced alone, also with the oxidized) still had the same result Rana
- [ccp4bb] protein cleavage rana ibd
- Re: [ccp4bb] protein cleavage Cynthia Kinsland
- Re: [ccp4bb] protein cleavage Bosch, Juergen
- Re: [ccp4bb] protein cleavage Cynthia Kinsland
- Re: [ccp4bb] protein cleavage VAN RAAIJ , MARK JOHAN
- Re: [ccp4bb] protein cleavage Sangeetha Vedula
- Re: [ccp4bb] protein cleavage SD Y
- Re: [ccp4bb] protein cleavage R. M. Garavito
- Re: [ccp4bb] protein cleavage D Bonsor
- [ccp4bb] protein cleavage rana ibd