[ccp4bb] Open call for Principal Researcher to be Leader of the Research Team of Horizon Europe ERA Chair project CryoEM@NOVA (deadline to apply: Oct 6, 2023)

[Ana Luísa Moreira de Carvalho]

Dear colleagues,


on behalf of Prof. Hartmut Luecke, ERA Chair of Horizon Europe project 
CryoEM@NOVA, from the NOVA University of Lisbon (Portugal), I bring your 
attention to the open call (text below) to hire, on a permanent basis, a 
Principal Researcher who will take the role of Leader of the Research Team in 
the 5-year project, started in January.
Please, share this information among all potentially interested.
Thank you.
Ana Luísa


Open Position: Principal Researcher



Job Type: Full Time

Position: Possibility of permanent contract

Application Starts: 14 Sep. 2023

Application Ends: 06 Oct. 2023


A call for a Principal Researcher position is available in the Horizon Europe - 
ERA Chair Group of Structural Biology and Drug Development lead by Prof. 
Hartmut Luecke (Cryo EM@NOVA , at the 
UCIBIO  Research Unit from the NOVA School of Science and 
Technology ).

 
A competitive candidate will have:

·  a Ph.D. degree in biochemistry, chemistry, molecular biology or a 
related area

·  a highly motivated individual with a strong track record and 
demonstrated experience in setting up a cryo EM laboratory;

·  knowledge of protein purification and biophysical and biochemical 
characterization of proteins and protein complexes, preferably including 
membrane proteins is required.

 
The Principal Researcher will assume the position of Leader of Research Team 
and play a leading role in implementing and coordinating the CryoEM@NOVA 
project funded by Horizon Europe!

 
For more detail visit the EURAXESS Portal: 
https://euraxess.ec.europa.eu/jobs/145817 

or the Public Notice (at NOVA School of Science and Technology): 
https://www.fct.unl.pt/sites/default/files/documentos/concursos/public_notice_17766.2023.pdf
 


Project website (at CORDIS): https://cordis.europa.eu/project/id/101087571 

Project website (at NOVA School of Science and Technology): 
https://sites.fct.unl.pt/cryoem-at-nova/ 








Ana Luísa Carvalho

Senior Researcher | UCIBIO-i4HB | XTAL - Macromolecular Crystallography
CiênciaID: 351C-DCB9-6912; http://docentes.fct.unl.pt/almc 

 
UCIBIO - Applied Molecular Biosciences Unit 
Associate Laboratory i4HB - Institute for Health and Bioeconomy 



Address:
Departamento de Química
Cristalografia de Raios-X (Gab. 6.34)
NOVA SCHOOL OF SCIENCE AND TECHNOLOGY | FCT NOVA
Universidade NOVA de Lisboa
Campus de Caparica | 2829-516 Caparica | Portugal
www.fct.unl.pt 
Tel: (+351) 21 2948300  Ext: 10940 (Gab); 11424 (Lab); 11427 (X-ray Diff. Lab)





Ana Luísa Carvalho

Senior Researcher | UCIBIO-i4HB | XTAL - Macromolecular Crystallography
CiênciaID: 351C-DCB9-6912; http://docentes.fct.unl.pt/almc 

 
UCIBIO - Applied Molecular Biosciences Unit 
Associate Laboratory i4HB - Institute for Health and Bioeconomy 



Address:
Departamento de Química
Cristalografia de Raios-X (Gab. 6.34)
NOVA SCHOOL OF SCIENCE AND TECHNOLOGY | FCT NOVA
Universidade NOVA de Lisboa
Campus de Caparica | 2829-516 Caparica | Portugal
www.fct.unl.pt 
Tel: (+351) 21 2948300  Ext: 10940 (Gab); 11424 (Lab); 11427 (X-ray Diff. Lab)






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Re: [ccp4bb] in memoriam of Raimond Ravelli

[Ana Luísa Moreira de Carvalho]

As ESRF users, and also meeting Raimond in many courses and congresses since 
the 90s’, here we are also very saddened by these news.
He was brilliant and very helpful.


Ana Luísa Carvalho

Senior Researcher | UCIBIO-i4HB | XTAL - Macromolecular Crystallography
CiênciaID: 351C-DCB9-6912; http://docentes.fct.unl.pt/almc 

 
UCIBIO - Applied Molecular Biosciences Unit 
Associate Laboratory i4HB - Institute for Health and Bioeconomy 



Address:
Departamento de Química
Cristalografia de Raios-X (Gab. 6.34)
NOVA SCHOOL OF SCIENCE AND TECHNOLOGY | FCT NOVA
Universidade NOVA de Lisboa
Campus de Caparica | 2829-516 Caparica | Portugal
www.fct.unl.pt 
Tel: (+351) 21 2948300  Ext: 10940 (Gab); 11424 (Lab); 11427 (X-ray Diff. Lab)




> On 6 Jul 2023, at 14:51, Pedro Matias  wrote:
> 
> Dear All,
> 
> The loss of Raymond is indeed a tragedy for all of us, not in the least 
> because he was still so young and with so much to give to Science.
> 
> I will always remember his cheerfulness and good nature, and keep fond 
> memories of his time at the ESRF where he was our local contact on many 
> occasions.
> 
> Pedro Matias
> 
> On 05/07/2023 19:54, a.perra...@nki.nl  wrote:
>> Dear all,
>> 
>> It is with profound sadness I announce the passing of Raimond Ravelli, one 
>> of most remarkable and beloved members of our scientific community.
>> 
>> Raimond first became widely known to the crystallographic community with his 
>> brilliant “strategy” program in the 90’s, which he developed as part of his 
>> PhD thesis. Later on, at the EMBL Grenoble and the ESRF, Raimond has been 
>> instrumental in the development of the ID14-EH4 beamline, having helped 
>> numerous colleagues to collect diffraction data and participating in many 
>> exciting scientific projects. At that time, Raimond also developed his keen 
>> interest in radiation damage, that influenced the ways that we collect and 
>> process X-ray diffraction data even today. Raimond came back to his home 
>> country, the Netherlands, first as an assistant professor at Leiden 
>> University, and then as associate professor at Maastricht University. During 
>> that transition he become an expert in cryo-electron microscopy. He 
>> made numerous contributions also to his new field, in such a broad spectrum 
>> of techniques, that it would be embarrassing to attempt to enumerate them 
>> here. I will only mention that one piece of his work even became a museum 
>> exhibit at the Leiden museum of natural history.
>> 
>> Raimond has been recently appointed Full Professor of Structural Biology, at 
>> Maastricht University, last November. His inauguration speech (Oratie) was 
>> simply remarkable. I warmly recommend to anyone to watch it at 
>> https://youtu.be/Gv3pKRp5S2Y?t=667  . It 
>> radiates his enthusiasm for science and is inspiring for all, in a way that 
>> only he himself knew how to do.
>> 
>> Raimond was an accomplished rowing athlete, a formidable cyclist, and a 
>> hiking enthusiast. 
>> 
>> For the many of us who have been his friends it has a been a privilege and 
>> an inspiration. Raimond, just 55, leaves behind him Maaike, an excellent 
>> scientist and colleague herself, and their kids Seppe and Noé. 
>> 
>> I have no words that could possibly explain how much he will be missed.
>> 
>> 
>> 
>> 
>> To unsubscribe from the CCP4BB list, click the following link:
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>> -- 
> Industry and Medicine Applied Crystallography
> Macromolecular Crystallography Unit
> ___
> Phones : (351-21) 446-9100 Ext. 1669
>  (351-21) 446-9669 (direct)
>  Fax   : (351-21) 441-1277 or 443-3644
> 
> email : mat...@itqb.unl.pt 
> 
> http://www.itqb.unl.pt/research/biological-chemistry/industry-and-medicine-applied-crystallography
>  
> 
> http://www.itqb.unl.pt/labs/macromolecular-crystallography-unit 
> 
> 
> Mailing address :
> Instituto de Tecnologia Quimica e Biologica António Xavier
> Universidade Nova de Lisboa
> Av. da República
> 2780-157 Oeiras
> PORTUGAL
> 
> ITQB NOVA, a great choice for your PhD
> https://youtu.be/de6j-aaTWNQ 
> 
> Master Programme in Biochemistry for Health
> https://youtu.be/UKstDCFjYI8 
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> 

Re: [ccp4bb] Can twinning be seen in the diffraction pattern?

[Ana Luísa Moreira de Carvalho]

Just a short note on this: I often see colleagues using the word “twinning" 
when referring to a crystal that is actually multiple (not single).

I think much confusion arises from this. For me, a twin crystal is the one that 
looks single under the microscope and only intensity statistics reveal that the 
diffraction comes from more than one crystal.

If a crystal looks multiple, i do not call it a twin. Am i being too meticulous 
on this?
Thanks!

> On 16 Mar 2021, at 13:31, Eleanor Dodson 
> <176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk> wrote:
> 
> You usually detect twinning most reliably from the intensity statistics - 
> CCP4I2 and Xtriage report those..
> Eleanor
> 
> On Tue, 16 Mar 2021 at 07:31, Marina Gárdonyi 
>  > wrote:
> Dear all,
> 
> thanks to all who helped me solving the question. You sent me a lot of  
> comments and information I have not taken into account.
> After reading all the answers, I have come to the conclusion that the  
> spots that are very close to each other come from the long cell axis  
> (57-57-160) and that twinning can probably not be seen in my case. I  
> should have mentioned that the diffraction images came from an  
> in-house x-ray machine, recorded with a 0.5 degree rotation range.
> 
> Thank you all again!
> 
> Kind regards,
> Marina
> 
> -- 
> Marina Gárdonyi
> 
> PhD Student, Research Group Professor Dr. Klebe
> 
> Department of Pharmaceutical Chemistry
> 
> Philipps-University Marburg
> 
> Marbacher Weg 6, 35032 Marburg, Germany
> 
> Phone: +49 6421 28 21392
> 
> E-Mail: marina@pharmazie.uni-marburg.de 
> 
> 
> http://www.agklebe.de/ 
> 
> 
> 
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Re: [ccp4bb] Micro/Macro crystal seeding experience

[Ana Luísa Moreira de Carvalho]

Hi Rafael,

have you considered crystal dehydration? 
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317743/ 

It’s not easy to implement, but high solvent content could be a reason why your 
crystals don’t diffract beyond 4A. Did you manage to get some unit cell 
parameters from this 4A diffraction and estimate the solvent content?
Good luck!

> On 17 Dec 2020, at 21:30, Rafael Marques  wrote:
> 
> Hello all, how are you doing? 
>  
> I have been working with two different proteins for 3 years. I was able to 
> purify them by IMAC and SEC, no problems at all, and I have got some crystals 
> of both. However, the best data I have got until now is around 4A. The 
> diffraction experiment was carried out several times at DLS. 
>  
> The crystals for each protein are different. One is really small whereas the 
> other one is a regular size (Images below). Some people have told me to try 
> microseeding and I did this, but with no success at all.
>  
> So I am asking here if there is someone experienced in this kind of 
> witchcraft that could give me a hand and also answer me these two things:
>  
> Have you ever had good size crystals that didn’t diffract beyond 4A and after 
> trying seeding you have got very good diffraction data (in these case with no 
> change concerning the crystal size)?
>  
> What is the best protocol that you have tried to increase the size of your 
> crystals and make them diffract better?
>  
> Many thanks in advance.
>  
> Best
>  
> <5A70957A7B3E4521AAC422B1E6B83034.png>
>  
> 
> Rafael Marques da Silva
> Mestrando em Física Biomolecular
> Universidade de São Paulo
>  
> Bacharel em Ciências Biológicas
> Universidade Federal de São Carlos
>  
> phone: +55 16 99766-0021
>  
>"A sorte acompanha uma mente bem treinada"
> 
>  
> 
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[ccp4bb] Challenges in Protein Crystallisation

[Ana Luísa Moreira de Carvalho]

Dear fellow crystallographers,

very much related to the recent discussion on the science behind 
crystallisation, we bring to your attention a Special Issue on "NOVEL 
STRATEGIES FOR IMPROVED PROTEIN CRYSTALLIZATION” from the MDPI journal 
Crystals: https://www.mdpi.com/journal/crystals/special_issues/novel_protein

This is a great opportunity to gather the expertises from many of you that have 
worked on unconventional crystallisation methods and/or in the theory behind 
these crystallisation methods. Short reviews or new research articles are 
welcome.

Due to the open access policy, the journal charges publication fees, but, 
please, contact us directly for the chance to get a 20% discount grant. The 
deadline for submissions is 31st December 2019. 

We're putting a great effort in gathering scientific testimonies of research in 
this field (and we’re glad that some colleagues of this bb have already agreed 
to collaborate - thank you!).
Our apologies for using this BB for advertising this Issue and thanks for any 
attention this may deserve. 

Best wishes,

Maria João Romão
Ana Luísa Carvalho


Watch out for this Special Issue from Crystals! 
The Call for Papers 
 is open.



Research Assistant Professor at UCIBIO@FCT-NOVA
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc 
http://sites.fct.unl.pt/xtal 
https://www.facebook.com/XtalNOVA
***




> On 24 Jul 2019, at 11:00, Hargreaves, David 
>  wrote:
> 
> Dear CCP4bb,
> 
> From my industrial perspective: The crystallisation bottleneck has probably 
> been fairly well addressed. High throughput screening using robotics and 
> around 20ul of protein per screen is common. There are lots of screens to try 
> even if they are rather redundant in their content. However, I feel there are 
> two things missing: high throughput protein production at microscale and 
> specific tools for construct design. Generally, industry is interested in the 
> druggable site which perhaps is only one domain of a multidomain target 
> protein. Getting a suitably robust crystal system often requires more than 
> one iteration of multiple construct design which in my experience is 
> something of a lottery. Some helpful design tools and an order of magnitude 
> increase in the number of constructs that can be delivered from protein 
> supply would help.
> 
> Best wishes,
> 
> David
> 
> 
> Dr. David Hargreaves
> Associate Principal Scientist
> _
> AstraZeneca
> Discovery Sciences, Structure & Biophysics
> Dr David Hargreaves.
> Office R1-09/Lab FL54
> AstraZeneca Darwin Building
> Unit 310
> Cambridge Science Park
> Milton Road
> Cambridge
> CB4 0WG
> 
> telephone: +441223223546
> 
> David.Hargreaves @astrazeneca.com
> 
> Please consider the environment before printing this e-mail
> 
> 
> 
> 
> AstraZeneca UK Limited is a company incorporated in England and Wales with 
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Re: [ccp4bb] Is it possible to fix the position of an atom during real space fitting in Coot?

[Ana Luísa Moreira de Carvalho]

You can find the tool “Fixed Atoms” in the Refinement Toolbar (the side menu). 
Just click Fix Atom and then click the atom(s) you wish to fix. It won’t move 
in real space refinement or in regularisation.
Cheers!

> On 13 Mar 2018, at 19:46, Steve Chou  wrote:
> 
> Dear Coot developers and CCP4 list subscribers,
> 
> The density of my map is not perfect. However, I know where a specific atom 
> should be located. Every time I did real space fitting, the atom and the 
> associated chemical groups in the whole ligand ran away. Is there a way to 
> fix the position of this atom, and let the force field drive the fitting of 
> the rest of the atoms in the ligand?
> Many thanks in advance!
> Steve
> 
> -- 
> Steve Chou
> 
> 

Research Assistant Professor at UCIBIO@REQUIMTE-FCT-NOVA
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc 
http://sites.fct.unl.pt/xtal 
https://orcid.org/-0002-3824-0240/print
***

[ccp4bb] Off topic: Anyone still using Oxford Cryosystems Cryostream 600 series?

[Ana Luísa Moreira de Carvalho]

Dear colleagues,


Anyone still using Oxford Cryosystems Cryostream 600 series?
We would like to know if there is interest in a computer program to control 
these units via a rs232 connection. We are implementing this in our home source.

For data collection, the program allows automated shutdown if no new images are 
collected for a specified period.

The data collection software in our home source has this functionality but it 
does not support these old units.  Since our controller is working fine, and is 
quite robust, we have no reason to replace it.

With this program we can leave data collection running overnight and the cryo 
shuts down automatically when finished. 

Please, let me know if this would be a useful feature to add to your X-ray 
facility. Reply privately, if you prefer.
Thanks and best wishes
Ana Luisa



Research Assistant Professor at UCIBIO@REQUIMTE-FCT-NOVA
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-NOVA
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc
http://sites.fct.unl.pt/xtal
https://www.facebook.com/XtalNOVA/
***
Single Crystal X-ray Structure Determination Service: 
http://www.dq.fct.unl.pt/en/single-crystal-x-ray-structure-determination



Research Assistant Professor at UCIBIO@REQUIMTE-FCT-NOVA
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-NOVA
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc
http://sites.fct.unl.pt/xtal
https://www.facebook.com/XtalNOVA/
***
Single Crystal X-ray Structure Determination Service: 
http://www.dq.fct.unl.pt/en/single-crystal-x-ray-structure-determination

Re: [ccp4bb] Unknown electron density blob

[Ana Luísa Moreira de Carvalho]

Just to add to this, in our group, we had a funny case where we found PEG 
around K:

https://drive.google.com/open?id=0B2DrnhrLgvwGSldLVl9pSEptakE
https://drive.google.com/open?id=0B2DrnhrLgvwGZlNiYTFoaktPUHc

Ana Luisa


> On 24 Jan 2017, at 17:36, Artem Evdokimov  wrote:
> 
> PEG. It wraps around K or R residues just like you are showing.
> 
> Artem
> www.harkerbio.com 
> "where every blob has candy inside"
> 
> On Jan 24, 2017 12:19 PM, "Uma Gabale" 
> <0ebb5dcf3eaa-dmarc-requ...@jiscmail.ac.uk 
> > wrote:
> Dear all,
> While refining a structure at 2.5 A resolution, we observed a 
> semi-circular/crescent shaped electron density blob as shown in the attached 
> picture. We have been unable to identify it so far, and would appreciate any 
> help in identification.
> The protein was expressed in E. coli BL21(DE3), purified on Ni-NTA followed 
> by gel filtration. The purification buffers included Tris and NaCl (no 
> detergent/ other ingredients except for imidazole for Ni-NTA). 
> Crystallization condition had HEPES and PEG3350; no cryoprotectant was used.
> The blob is surrounded by residues Trp, Thr, Gln, Arg, and Phe.
> Thanks and regards,
> Uma.
> 
> --
> Uma Gabale, PhD
> Research Associate
> Molecular and Cellular Biochemistry
> Indiana University Bloomington

Research Assistant Professor at UCIBIO@REQUIMTE-FCT-NOVA
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc 
http://sites.fct.unl.pt/xtal
https://www.facebook.com/XtalNOVA/
***
Single Crystal X-ray Structure Determination Service: 
http://www.dq.fct.unl.pt/en/single-crystal-x-ray-structure-determination

[ccp4bb] Post-Doctoral position in Caparica, Portugal

[Ana Luísa Moreira de Carvalho]

ANNOUNCEMENT FOR A POST-DOCTORAL POSITION IN CAPARICA, PORTUGAL



The Macromolecular Crystallography Group from FCT (Universidade Nova de Lisboa, 
UNL) in Caparica is seeking a motivated researcher to work as a post-doctoral 
fellow, in the project Modern Structural Biology: Resources for the 
advancement of in-house X-ray Crystallography, Ref. RECI/BBB-BEP/0124/2012 
financed by the Portuguese Science and Technology Foundation.


Work will be carried out in the Macromolecular Crystallography Lab 
(http://sites.fct.unl.pt/xtal) at FCT-UNL, Caparica (www.fct.unl.pt) at the 
research Unit UCIBIO (www.ucibio.pt).


The candidates should hold a PhD in Chemistry, Biochemistry, or a related 
field, and a strong experience in X-ray Crystallography.

The hired researcher will be involved in some of the Structural Biology 
projects running in the group (http://sites.fct.unl.pt/xtal/pages/research) but 
the possibility of developing own research is also encouraged. He/she will also 
be dedicated to the operation and management of the Lab's X-ray diffractometers 
and associated equipment and shall give support to the small molecules' 
structure solution service.

Preference will be given to candidates with experience in protein 
crystallography as well as small molecule crystallography. Although not 
mandatory, it can constitute an additional advantage the experience in protein 
expression and purification.

A starting contract for 6 months will be offered, starting in June 2015, with 
the possibility of renovation up to a maximum period of 36 months (through a 
post-doctoral grant from the Portuguese Science Foundation). The stipend 
corresponds to EUR1495 (free of taxes).


Applications are open until the 4th of May.

To apply, candidates should send a motivation letter, curriculum vitae and 
names and emails of three referees to Dr Ana Luisa Carvalho: a...@fct.unl.pt.



To know more about our campus, please visit: http://www.fct.unl.pt/en

To know more about the region of Lisbon,visit: 
http://www.visitlisboa.com/LisbonRegion.aspx

-- 
Maria João Romão
Presidente do Departamento / Head of Department
Director of UCIBIO@REQUIMTE, Departamento Química/ Faculdade de Ciências e 
Tecnologia 
Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
Tel: +351-212948345; Secretary: +351-212948575 Fax: +351-212948550
e-mail: maria.ro...@fct.unl.pt
www.ucibio.pt www.requimte.pt/ucibio/people/mjrfctunlpt
http://sites.fct.unl.pt/xtal
-

 

[ccp4bb] INSTRUCT Training Course announcement: ISBio2015 in Caparica, Portugal

[Ana Luísa Moreira de Carvalho]

Dear CCP4BB community,


we are pleased to announce that the period for applications to attend the 
second edition of the INSTRUCT-COST Training Course ISBio2015 
http://xtal.dq.fct.unl.pt/PosterISBio-2015.pdf is open until June 15. 


Integrative Structural Biology tools for the study of protein-ligand 
interactions

The course will take place at FCT-Universidade NOVA de Lisboa, in the campus of 
Caparica, Portugal, from 13-19 July 2015 

COURSE DETAILS  REGISTRATION:  http://eventos.fct.unl.pt/isbio 
http://eventos.fct.unl.pt/isbio

http://www.structuralbiology.eu/support/whats-on/calendar-events/isbio2015-integrative-structural-biology-tools-study-protein-ligand
 
http://www.structuralbiology.eu/support/whats-on/calendar-events/isbio2015-integrative-structural-biology-tools-study-protein-ligand

APPLICATION DEADLINE: June 15, 2014 (attendance is limited to 18 participants; 
PhD students and young Post-docs will be given priority) 

OBJECTIVE OF THE COURSE: to illustrate the added value of a structural biology 
approach to the study of protein-ligand interactions by combining core 
methodologies, X-ray Crystallography, NMR, EPR, Carbohydrate Microarrays, ITC 
and Molecular Modeling from a hands-on perspective, giving emphasis to their 
limitations and complementarities.
To achieve this, and to better illustrate the integrative context, the students 
will receive training by working in a study-case, a protein that recognizes and 
binds to carbohydrates, which will be the focus of the several complementary 
methodologies. Besides the hands-on training there will be theory lectures 
where the main theoretical aspects of each technique will be explained always 
with an emphasis on the complementarity with the other techniques.

APPLICATIONS AND COURSE FEE: The detailed registration info and the online 
application form are available at 
http://eventos.fct.unl.pt/isbio/pages/registration-info 
http://eventos.fct.unl.pt/isbio/pages/registration-info


Fee is €450 and includes attendance, course documentation, coffee breaks (all 
course days), lunches (all course days), course dinner (date to be announced) 
and accommodation (single room inc. breakfast in Lisboa Almada Hotel 
http://www.lisboaalmadahotel.com/en/hotel-overview.html; check-in: July 12; 
check-out: July 19).

SUPPORTED BY: INSTRUCT, COST, UCIBIO-REQUIMTE@FCT-UNL

Please, forward this information to anyone who might be interested. 

We are looking forward to welcome you in Caparica (and the beaches nearby...)

Best regards, 
The Organizers
Ana Luisa Carvalho  Eurico Cabrita






Assistant Researcher of Associate Lab UCIBIO@REQUIMTE-FCT-UNL
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc/biocv http://docentes.fct.unl.pt/almc/biocv
http://sites.fct.unl.pt/xtal http://sites.fct.unl.pt/xtal
***

Visit the Portuguese Centre for Integrated Structural Biology: 
http://xtal.dq.fct.unl.pt/PCISBIO http://xtal.dq.fct.unl.pt/PCISBIO and learn 
how you can benefit from all the infrastructures and skills!

And join us in the IYCr2014 http://xtal.dq.fct.unl.pt/iycr2014 celebrations 
in Caparica!

[ccp4bb] INSTRUCT Training Course announcement: ISBio2014 in Caparica, Portugal

[Ana Luísa Moreira de Carvalho]

Dear CCP4BB community,


we inform that the deadline for applications to the INSTRUCT Training Course 
ISBio2014 has now been extended to June 10. 


Integrative Structural Biology tools for the study of protein-ligand 
interactions

The course will take place at FCT-Universidade NOVA de Lisboa, in the campus of 
Caparica, Portugal, from 6-12 October 2014 

COURSE DETAILS  REGISTRATION:  http://eventos.fct.unl.pt/isbio2014

http://www.structuralbiology.eu/support/whats-on/calendar-events/integrative-structural-biology-tools-study-protein-ligand

APPLICATION DEADLINE: June 10, 2014 (attendance is limited to 16 participants; 
PhD students and young Post-docs will be given priority) 

OBJECTIVE OF THE COURSE: to illustrate the added value of a structural biology 
approach to the study of protein-ligand interactions by combining four core 
methodologies, X-ray Crystallography, NMR, Carbohydrate Microarrays and ITC 
from a hands-on perspective, giving emphasis to their limitations and 
complementarities.
To achieve this, and to better illustrate the integrative context, the students 
will receive training by working in a study-case, a protein that recognizes and 
binds to carbohydrates, which will be the focus of the several complementary 
methodologies. Besides the hands-on training there will be theory lectures 
where the main theoretical aspects of each technique will be explained always 
with an emphasis on the complementarity with the other techniques.

APPLICATIONS AND COURSE FEE: The detailed registration info and the online 
application form are available at 
http://eventos.fct.unl.pt/isbio2014/pages/registration-info


Fee is €350 and includes attendance, course documentation, coffee breaks (all 
course days), lunches (all course days), course dinner (Oct 7) and 
accommodation (single room inc. breakfast in Lisboa Almada Hotel; check-in: Oct 
5; check-out: Oct 12).

SUPPORTED BY: INSTRUCT, REQUIMTE-FCT-UNL, CIISA-FMV-UL

Please, forward this information to anyone who might be interested. 

We are looking forward to welcome you in Caparica (and the beaches nearby...)

Best regards, 
The Organizers
Ana Luisa Carvalho  Eurico Cabrita



Assistant Researcher of Associate Lab REQUIMTE/CQFB@FCT-UNL
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc/biocv
http://xtal.dq.fct.unl.pt/
***

INSTRUCT Training Course in Caparica, from 6 to 12 October: ISBio2014  
(registration open until June, 1st) 

Visit the Portuguese Centre for Integrated Structural Biology: 
http://xtal.dq.fct.unl.pt/PCISBIO and learn how you can benefit from all the 
infrastructures and skills!

And join us in the IYCr2014 celebrations in Caparica!

[ccp4bb] INSTRUCT Training Course announcement: ISBio2014 in Caparica, Portugal

[Ana Luísa Moreira de Carvalho]

2nd Call for applications to the INSTRUCT Training Course 

Integrative Structural Biology tools for the study of protein-ligand 
interactions

The course will take place at FCT-Universidade NOVA de Lisboa, in the campus of 
Caparica, Portugal, from 6-12 October 2014 

COURSE DETAILS  REGISTRATION:  http://eventos.fct.unl.pt/isbio2014

http://www.structuralbiology.eu/support/whats-on/calendar-events/integrative-structural-biology-tools-study-protein-ligand

APPLICATION DEADLINE: June 1, 2014 (attendance is limited to 16 participants; 
PhD students and young Post-docs will be given priority) 

OBJECTIVE OF THE COURSE: to illustrate the added value of a structural biology 
approach to the study of protein-ligand interactions by combining four core 
methodologies, X-ray Crystallography, NMR, Carbohydrate Microarrays and ITC 
from a hands-on perspective, giving emphasis to their limitations and 
complementarities.
To achieve this, and to better illustrate the integrative context, the students 
will receive training by working in a study-case, a protein that recognizes and 
binds to carbohydrates, which will be the focus of the several complementary 
methodologies. Besides the hands-on training there will be theory lectures 
where the main theoretical aspects of each technique will be explained always 
with an emphasis on the complementarity with the other techniques.

APPLICATIONS AND COURSE FEE: The detailed registration info and the online 
application form are available at 
http://eventos.fct.unl.pt/isbio2014/pages/registration-info


Fee is €350 and includes attendance, course documentation, coffee breaks (all 
course days), lunches (all course days), course dinner (Oct 7) and 
accommodation (single room inc. breakfast in Lisboa Almada Hotel; check-in: Oct 
5; check-out: Oct 12).

SUPPORTED BY: INSTRUCT, REQUIMTE-FCT-UNL, CIISA-FMV-UL

Please, forward this information to anyone who might be interested. 

We are looking forward to welcome you in Caparica (and the beaches nearby...)

Best regards, 
The Organizers
Ana Luisa Carvalho  Eurico Cabrita



Assistant Researcher of Associate Lab REQUIMTE/CQFB@FCT-UNL
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext: Gab: 10940; Lab: 10962; X-ray Lab: 10915)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc/biocv
http://xtal.dq.fct.unl.pt/
***

INSTRUCT Training Course in Caparica, from 6 to 12 October: ISBio2014  
(registration open until June, 1st) 

Visit the Portuguese Centre for Integrated Structural Biology: 
http://xtal.dq.fct.unl.pt/PCISBIO and learn how you can benefit from all the 
infrastructures and skills!

And join us in the IYCr2014 celebrations in Caparica!

[ccp4bb] INSTRUCT Training Course announcement: ISBio2014 in Caparica, Portugal

[Ana Luísa Moreira de Carvalho]

2nd Call for applications to the INSTRUCT Training Course 

Integrative Structural Biology tools for the study of protein-ligand 
interactions

The course will take place at FCT-Universidade NOVA de Lisboa, in the campus of 
Caparica, Portugal, from 6-12 October 2014 

COURSE DETAILS  REGISTRATION:  http://eventos.fct.unl.pt/isbio2014

http://www.structuralbiology.eu/support/whats-on/calendar-events/integrative-structural-biology-tools-study-protein-ligand

APPLICATION DEADLINE: June 1, 2014 (attendance is limited to 16 participants; 
PhD students and young Post-docs will be given priority) 

OBJECTIVE OF THE COURSE: to illustrate the added value of a structural biology 
approach to the study of protein-ligand interactions by combining four core 
methodologies, X-ray Crystallography, NMR, Carbohydrate Microarrays and ITC 
from a hands-on perspective, giving emphasis to their limitations and 
complementarities.
To achieve this, and to better illustrate the integrative context, the students 
will receive training by working in a study-case, a protein that recognizes and 
binds to carbohydrates, which will be the focus of the several complementary 
methodologies. Besides the hands-on training there will be theory lectures 
where the main theoretical aspects of each technique will be explained always 
with an emphasis on the complementarity with the other techniques.

APPLICATIONS AND COURSE FEE: The detailed registration info and the online 
application form are available at 
http://eventos.fct.unl.pt/isbio2014/pages/registration-info


Fee is €350 and includes attendance, course documentation, coffee breaks (all 
course days), lunches (all course days), course dinner (Oct 7) and 
accommodation (single room inc. breakfast in Lisboa Almada Hotel; check-in: Oct 
5; check-out: Oct 12).

SUPPORTED BY: INSTRUCT, REQUIMTE-FCT-UNL, CIISA-FMV-UL

Please, forward this information to anyone who might be interested. 

We are looking forward to welcome you in Caparica (and the beaches nearby...)

Best regards, 
The Organizers
Ana Luisa Carvalho  Eurico Cabrita



Assistant Researcher of Associate Lab REQUIMTE/CQFB@FCT-UNL
***
Biologia Estrutural - Cristalografia de Raios-X (Gab 6.34)
Dep. Quimica, FCT-UNL
2829-516 Caparica
Portugal
Phone: 00351212948300 (ext. 10940)
Fax: 00351212948550
http://docentes.fct.unl.pt/almc/biocv
http://xtal.dq.fct.unl.pt/
***

Post-Doctoral opportunity: 
http://xtal.dq.fct.unl.pt/PCISBIO/Job_announcements.html

INSTRUCT Training Course in Caparica, from 6 to 12 October: ISBio2014  
(registration open until June, 1st) 

Visit the Portuguese Centre for Integrated Structural Biology: 
http://xtal.dq.fct.unl.pt/PCISBIO and learn how you can benefit from all the 
infrastructures and skills!

And join us in the IYCr2014 celebrations in Caparica!