[ccp4bb] Insoluble ligand?

2011-09-16 Thread Chaudhary, Ritcha
Hi All I have a ligand that is dissolved in 100% DMSO. The ligand crashes out even when diluted to 20% final DMSO concentration in the protein buffer. I have heard of people using detergents such as n octyl Beta D glucoside to keep the ligand solubilized at lower DMSO concentrations. Can

[ccp4bb] peptide crystallization question

2011-09-06 Thread Chaudhary, Ritcha
Dear all I am interested in crystallizing a 5 residue peptide. I have no prior experience in this field although I have crystallized larger proteins (5--60 kDa). Do people use regular screens used in macromolecular crystallization such as Hamptons, wizards etc? Any suggestions are greatly