Argonne National Laboratory has the following job offer:
Requisition Number: 405283
Position Title: Postdoctoral Appointee
Career Level: 700
Division: ASD-Accelerator Systems Division
Location: Lemont, IL
Offsite Work Location: ANL
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Hours of Work: 40
The
Dear all,
I'd like to draw your attention to the beamline scientist position
currently open at the Structural Biology Center, ANL.
Job ID: 403448
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Hi,
The Lys-PLP adduct looks like a perfectly happy internal aldimine. I do
not see any indication of a covalent bond between PLP and the density in
question indicating any other intermediate. Which, to my knowledge,
would be rather hard to get accidentally. I do not know distances
between these
Hi Reza,
Check the following reference:
Cryoprotection properties of salts of organic acids: a case study for a
tetragonal crystal of HEW lysozyme.
Bujacz G, Wrzesniewska B, Bujacz A.
Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):789-96.
Cheers,
Karolina
W dniu 2014-12-01
4M NaCl should work too. It worked for the conditions with 1.8 - 2.0 M
NaCl.
Karolina
W dniu 2014-02-19 06:38, Mooers, Blaine H.M. (HSC) napisał(a):
For crystals grown out of a 2 uL drop of 1.2-1.8 M LiSO4 or 1.6-2.4 M AmmSO4,
we do in situ cryoprotection with sodium malonate. We add
Hi all,
I'm working with a protein that appears to be a dimer in solution, on
SEC in runs as 24 kDa, while the actual mass of a dimer is 30. And I am
trying to figure out which dimer is the biological one (it is a
regulatory protein but details are uknown). The crystal structure gives
me a
Hello everybody,
I'm looking for small proteins (up to 150 aa) that are known to express
reasonably in E. coli, are soluble and folded but failed to crystallize.
Can you provide some examples, especially of proteins that might be
interesting for a broad community?
Thanks,
Karolina
You may want to have a look at 3ur7 and 3ur8. I have also another example
of a glycohydrolytic enzyme with an N-terminal His-tag sitting in the
active site of a symmetry-related molecule (not deposited yet).
Karolina
On 27/6/2012, Brad Bennett bradbennet...@gmail.com wrote:
I think it was an
Hi all,
I have a ligand-corresponding density which I cannot identify:
http://s1194.photobucket.com/albums/aa376/dziuba22/?action=viewcurrent=coot1.png
Here is the story. I collected a couple of datasets, three of them belong
to methylated protein and one to non-methylated. The protein is
