[ccp4bb] Structural Biology Post available at Evotec

[McEwan, Paul]

Dear All,
A position is now available at EVOTEC for a Senior Scientist in Structural 
Biology. Please do not email me directly about this position but use the link 
below for applications.

Best regards,
Paul

https://evotecgroup.wd3.myworkdayjobs.com/en-US/Evotec_Career_Site/job/Abingdon-Evotec/Senior-Scientist_REQ-00835

Evotec is a global, publicly listed biotechnology company with more than 2,700 
employees at 13 sites, our headquarters are located in Hamburg. We do research 
on innovative approaches to drug discovery and development for and with leading 
pharmaceutical and biotechnology companies and academic institutions. By 
assembling top-class scientists, state-of-the-art technologies as well as 
substantial experience and expertise in key therapeutic areas including 
neuroscience, diabetes and complications of diabetes, pain and inflammation, 
oncology and infectious diseases, Evotec has established a unique leading 
position.

*   Required knowledge, skills and abilities:

  *   Extensive knowledge in protein biochemistry and macromolecular 
crystallography
  *   Experience with protein purification
  *   Protein engineering, bioinformatics and molecular biology (Construct 
design and cloning strategy)
  *   Familiar with Biophysic techniques (DSF, ITC...)
  *   A working knowledge and practical experience with either E. coli, insect 
or mammalian expression systems
  *   The successful candidate will have experience working in 
multi-disciplinary teams
  *   Experience collaborating with scientists in external organisations
Responsibilities includes:

  *   Reading scientific literature relevant to the project
  *   Supporting a project team by designing and ordering constructs to 
initiate the project
  *   Adapting scientific knowledge to resolve problems encountered in 
achieving defined goals with assistance from line manager or other team members 
to meet internal and project deadlines
  *   Preparing reports, presenting data, and collaborating with other 
scientists at Evotec
  *   Presenting own work at project technical meetings, and department 
technical meetings when required
  *   Demonstrating full command of all techniques and tools required for 
assigned responsibilities within the given project
  *   Ensuring safe working practices of themselves and any direct reports
  *   Complying fully with the company safety policy (including COPs and SOPs) 
when performing experiments
  *   Adhering to Lab Notebook guidelines regarding accurate documentation of 
lab experiments
Desirable Knowledge, Skills and Abilities:

  *   Experience in Cryo-EM
  *   Self-motivated, enthusiastic, cooperative and reliable
  *   Knowledge of basic laboratory techniques, and understanding of the 
science underlying the techniques and experiments
  *   Experience of working in a lab environment
  *   The ability to adapt to changing demands whilst operating under pressure 
to deadlines
  *   The ability to work accurately and with attention to detail
  *   Good interpersonal skills and the ability to work well with other 
scientific staff
  *   The ability to work well independently, and as part of a team
  *   Good organisational and time management skills
  *   Good verbal communication skills
  *   Ability to maintain excellent laboratory records
  *   Computer proficiency
Education & Experience:

  *   PhD degree in Structural Biology, Biochemistry or related area


[cid:image002.png@01D41934.D23B4340]
Paul McEwan, Ph.D.
Principal Scientist, Structural Biology
+44 (0)1235 83 8802  (Direct)
+44 (0)1235 86 3139  (Fax)
paul.mce...@evotec.com
www.evotec.com

Evotec (UK) Ltd.
114 Innovation Drive,
Milton Park, Abingdon
Oxfordshire OX14 4RZ, UK


STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


https://www.evotec.com/en/about/site-information/data-protection-uk

Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.



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[ccp4bb] Position Available at Evotec

[McEwan, Paul]

Dear All,
Please note I've had several emails from prospective applicants regarding 
failure of our weblink on the advert.

Could all applicants please repost applications through this weblink below:

https://evotecgroup.wd3.myworkdayjobs.com/Evotec_Career_Site


Best wishes,
Paul..
[cid:image002.png@01D41934.D23B4340]
Paul McEwan, Ph.D.
Principal Scientist, Structural Biology
+44 (0)1235 83 8802  (Direct)
+44 (0)1235 86 3139  (Fax)
paul.mce...@evotec.com
www.evotec.com

Evotec (UK) Ltd.
114 Innovation Drive,
Milton Park, Abingdon
Oxfordshire OX14 4RZ, UK


STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


https://www.evotec.com/en/about/site-information/data-protection-uk

Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.



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[ccp4bb] 2 crystallography posts available at Evotec

[McEwan, Paul]

Dear All,
There are two senior scientist posts available at Evotec in Abingdon, UK. 
Please do not contact me directly for application, please use the online 
recruitment weblink at the base of this email.




Evotec is a leader in the discovery and development of novel small molecule 
drugs with operational sites in Europe and the US. The Company has built 
substantial drug discovery expertise and an industrialised platform that can 
drive new innovative small molecule compounds into the clinic. In addition, 
Evotec has built a deep internal knowledge base in the treatment of diseases 
related to anti-infective, neuroscience, pain, oncology, inflammation and 
metabolic diseases. Leveraging these skills and expertise the Company intends 
to develop best-in-class differentiated therapeutics and deliver superior 
science-driven discovery alliances with pharmaceutical and biotechnology 
companies.
We are looking for highly motivated individuals for our Abingdon site to work 
as a
Senior Scientist, Structural Biology, X-Ray Crystallography (2 x 12-month 
temporary contract)
Evotec (UK) Ltd is currently seeking to add to our Structural Biology 
Department.  The group works closely with our Discovery Chemistry Department 
and with clients to develop novel small molecule drugs.  The group is at the 
forefront of new science and technology, and is seeking to expand as business 
needs grow.  Our site is located less than 4 miles from Diamond Light Source 
and eBIC national facilities for access to synchrotron radiation and Cryo 
Electron Microscopy respectively to support our high-end analysis of samples.
The position is initially temporary, for 12 months duration, but may become 
permanent dependant on business need.
Required Knowledge, Skills and Abilities:

  *   Expertise in X-ray crystallography from crystal preparation and handling, 
data collection through to analysis of structures
  *   Experience in gene design and protein purification
  *   Self-motivated, enthusiastic, cooperative and reliable
  *   Knowledge of basic laboratory techniques, and understanding of the 
science underlying the techniques and experiments
  *   Experience of working in a laboratory environment
  *   The ability to adapt to changing demands whilst operating under pressure 
to deadlines
  *   The ability to work accurately and with attention to detail
  *   Good interpersonal skills and the ability to work well with other 
scientific staff
  *   The ability to work well independently, and as part of a team
  *   Good organisational and time management skills
  *   Good verbal communication skills
  *   Ability to maintain excellent laboratory records
  *   Computer proficiency
Responsibilities may include:

  *   Supporting a project team by conducting experiments with defined, agreed 
objectives and timelines
  *   Contributing to the design, execution and interpretation of experiments 
in a project team to accomplish project goals
  *   Adapting scientific knowledge to resolve problems encountered in 
achieving defined goals with assistance from line manager or other team members 
to meet internal and project deadlines
  *   Preparing reports of findings, presenting data, and collaborating with 
other scientists at Evotec
  *   Presenting own work at project technical meetings, and department 
technical meetings when required
  *   Demonstrating full command of all techniques and tools required for 
assigned responsibilities within the given project
  *   Ensuring safe working practices of themselves and any direct reports
  *   Complying fully with the company safety policy (including COPs and SOPs) 
when performing experiments
  *   Reading scientific literature relevant to the project
  *   Adhering to Lab Notebook guidelines regarding accurate documentation of 
lab experiments
Desirable Knowledge, Skills and Abilities:

  *   Membrane protein expertise
  *   Cryo-Electron Microscopy sample preparation and data analysis
  *   Fragment based drug discovery
Education and Experience:


  *   PhD in Structural Biology, Biochemistry or related area



https://recruitment.evotec.com/VacancyDetails.aspx?FromSearch=True=QSAukis1tlc==738

best wishes,
Paul..
[cid:image002.png@01D41934.D23B4340]
Paul McEwan, Ph.D.
Principal Scientist, Structural Biology
+44 (0)1235 83 8802  (Direct)
+44 (0)1235 86 3139  (Fax)
paul.mce...@evotec.com
www.evotec.com

Evotec (UK) Ltd.
114 Innovation Drive,
Milton Park, Abingdon
Oxfordshire OX14 4RZ, UK


STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. 

[ccp4bb] Group Leader Position available at Evotec

[McEwan, Paul]

Dear All,
Evotec is expanding and is looking to recruit a Group Leader.

Please do not contact me directly but liaise with the Human Resources team 
though our website.

Best wishes,
Paul..

AB GL SB Sept18 - Group Leader, Structural Biology

Posted: 21/09/2018 15:08

Start Date: Not Available

Salary: competitive

Location: Abingdon

Level: Professional


Deadline: 19/10/2018 23:59

Hours: 40.00

Benefits: standard

Job Type: Full Time, Permanent



Evotec is a leader in the discovery and development of novel small molecule 
drugs with operational sites in Europe and the US. The Company has built 
substantial drug discovery expertise and an industrialised platform that can 
drive new innovative small molecule compounds into the clinic. In addition, 
Evotec has built a deep internal knowledge base in the treatment of diseases 
related to neuroscience, pain, oncology, inflammation and metabolic diseases. 
Leveraging these skills and expertise the Company intends to develop 
best-in-class differentiated therapeutics and deliver superior science-driven 
discovery alliances with pharmaceutical and biotechnology companies.
We are looking for a talented and highly motivated
Group Leader (Structural Biology)
Reporting to the SVP Protein Sciences, and based at our laboratories on Milton 
Park, in Abingdon, Oxfordshire, you will be a scientist with structural biology 
expertise with a background in protein science, X-ray crystallography or single 
particle cryo-EM.
The Protein Science team consists of 35 members of staff: 20 in recombinant 
protein production and characterisation; and 15 in X-ray crystallography, 
cryo-EM and other biophysics techniques. You will drive the scientific 
direction of the team, enhancing the future role of structural biology in drug 
discovery projects.  Excellent leadership skills and high motivation to 
contribute to integrated multidisciplinary teams are a must, as is an 
understanding for markets' and customers' needs to identify additional business 
opportunities.  A key role will be develop the members of the team.  A focus on 
end users' needs with a mind-set for integrity and openness are essential 
combined with an eye for detail, driving forward operational efficiency.
Required Knowledge, Skills and Abilities:

  *   Knowledge of the science underlying biology/biochemistry techniques and 
experiments
Responsibilities may include:

  *   Effective leadership of the Structural Biology team in Abingdon in order 
to achieve project goals within timelines and budget
  *   Drives and develops the group scientific and operational strategy to 
promote strong collaborative and in-house research programs
  *   Leading the development program for a diverse research team as it expands 
into new scientific areas, such as single particle Cryo-EM
  *   Responsible for the development of the members of the team; directly 
responsible for performance management, career development etc
  *   Build and maintain professional working relationships with clients to 
agree and influence scientific direction and project plans and ensure 
successful outcomes for both parties
  *   Effective communication of issues, changes of plan and progress; both 
externally (client) and internally (within teams and to senior management).
  *   Assists in the development and management of budgets; allocates 
resources, monitors project costs
  *   Supports activities such as proposal writing, business development 
activities and recruitment
Desirable Knowledge, Skills and Abilities:

  *   Experience in a drug discovery environment working on projects from 
target validation through to preclinical development candidate stage

  *   Strong leadership and management skills, and demonstrated success of 
leading a group of scientists
  *   Previous experience of working in a commercial environment; the 
successful candidate will have the ability to an understand the markets' and 
customers' needs to identify additional business opportunities
  *   High level of engagement, motivation and initiative to work comfortably 
and collaborate in a matrix environment
  *   Excellent interpersonal, technical, and communication skills to be an 
effective member on cross-functional teams as well as project management skills 
for timeline tracking and resource planning
Education and Experience:

  *   PhD (or equivalent) in structural biological sciences with demonstrated 
accomplished research experience
  *   At least 5 years of exposure to laboratory/practical work in a research 
environment; track-record of independent scientific achievement and 
demonstrated ability to work in interdisciplinary team setting

If you are interested in applying for this position, please visit Evotec's 
Career Page at https://recruitment.evotec.com/home.aspx
Closing date for applications 19th October 2018


[cid:image002.png@01D41934.D23B4340]
Paul McEwan, Ph.D.
Principal Scientist, Structural Biology
+44 (0)1235 83 8802  (Direct)
+44 (0)1235 86 

[ccp4bb] X-ray system available

[McEwan, Paul]

Dear CCP4bb readers,
We are in the process of decommissioning our in-house X-ray source and would 
like to offer it to the community. The device is showing its age based on the 
technology, but hopefully somebody could make use of it. In brief, the 
equipment is a Rigaku 007-HF generator with two Raxis4++ image plate detectors 
and computers for each of the ports. It is also equipped with a water to air 
chiller system to cool the anode.

If you are interested please drop myself and John Barker an email:
paul.mce...@evotec.com
john.bar...@evotec.com

I would note that Evotec offers this equipment on an as is basis and interested 
parties would be responsible for transportation and associated costs.

Best wishes,
Paul..
[cid:image002.png@01D41934.D23B4340]
Paul McEwan, Ph.D.
Principal Scientist, Structural Biology
paul.mce...@evotec.com
www.evotec.com

Evotec (UK) Ltd.
114 Innovation Drive,
Milton Park, Abingdon
Oxfordshire OX14 4RZ, UK


STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Job Opportunity

[McEwan, Paul]

Dear All,
I have an opportunity for somebody to join my team in structural biology at 
Evotec. All applications must be made through our website and please address 
any questions regarding the application process to 
humanresources-abing...@evotec.com


https://jobs.newscientist.com/job/1401624324/senior-scientist-structural-biology-oxford/

https://recruitment.evotec.com/home.aspx

Best regards,
Paul..
[EvotecLogoMail]
Paul A. McEwan Ph.D.
Principal Scientist, Structural Biology
+44. (0)1235 838802
+44. (0)1235 863139 (Fax)
paul.mce...@evotec.com
www.evotec.com

Evotec (UK) Ltd.
114 Innovation Drive
Milton Park
Abingdon
Oxfordshire
OX14 4RZ  (United Kingdom)


STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.

Re: [ccp4bb] how to reduce protein solubility

[McEwan, Paul]

Hi Francesca,
You could try using reductive methylation, this has always lead to a loss of 
solubility (and protein!) in my experience­. Jena bioscience and Hampton 
research sell kits.

Best regards,
Paul..
[EvotecLogoMail]
Paul A. McEwan Ph.D.
Principal Scientist, Structural Biology
+44. (0)1235 838802
+44. (0)1235 863139 (Fax)
paul.mce...@evotec.commailto:paul.mce...@evotec.com
www.evotec.comhttp://www.evotec.com/

Evotec (UK) Ltd.
114 Innovation Drive
Milton Park
Abingdon
Oxfordshire
OX14 4RZ  (United Kingdom)

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of David 
Briggs
Sent: 17 February 2015 08:12
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to reduce protein solubility


Hi Francesca,

Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of 
things less soluble in my hands.

Dave

On Tue, 17 Feb 2015 04:34 Mattiroli,Francesca 
francesca.mattir...@colostate.edumailto:francesca.mattir...@colostate.edu 
wrote:
Hi all,

I am struggling with a protein complex that is too soluble. I have reached 
about 20 mg/ml but I still observe very little precipitation (clear drops in 
90-95% of the tested conditions). The proteins are expressed in insect cells 
and going to higher concentration is not easily achievable.
I have tried different buffer conditions (salt concentration and pH) and I am 
testing temperatures. I am at a loss with what to try next.
Do you think PTMs (phosphorylation, acetylation) might be causing this?
Any input on how to decrease solubility?

Thank you very much in advance,

Francesca


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.

[ccp4bb] Industrial Protein crystallographer post available

[McEwan, Paul]

Dear CCP4bb members,
We have an exciting opportunity for somebody to become a member of our 
expanding structural biology unit to support development of new drugs, 
biopharmaceuticals and biotechnologies.

Please only apply via the careers page on our website 
www.evotec.comhttp://www.evotec.com

Best regards,
Paul..



[evotec_logo_RGB]

Protein Crystallographer


Location: Oxfordshire, UK
Full time; initial 6-month temporary contract, which may be extended or made 
permanent
Salary: Competitive

Evotec (UK) Ltd is currently seeking X-Ray Crystallographers for our Structural 
Biology Department based in Oxfordshire. X-Ray Crystallography works closely 
with our Discovery Chemistry Department and with clients to develop novel small 
molecule drugs. The group is at the forefront of new science and technology, 
and is seeking to support its continued expansion.

The successful candidates will be part of the Structural Biology group 
responsible for crystallisation and structure determination and interpretation 
of protein and protein-ligand complex structures.

Desirable Knowledge, Skills and Abilities:



* Practical laboratory skills and a willingness to learn

* Excellent communication and interpersonal skills



For appointment at a higher level:



* Knowledge of state of the art crystallographic methods

* Skills in molecular biology and protein purification

* Expertise in protein-ligand complex crystallisation problems would be an 
advantage though not essential

* Client interaction and project management skills

Education and Experience:


* Degree in Chemistry, Biochemistry, Molecular Biology or Biophysics or 
equivalent with a strong interest in protein structure



For appointment at a higher level:



* PhD qualified or equivalent in Chemistry, Biochemistry, Molecular Biology or 
Biophysics

* Experience in protein X-ray crystallography

* Candidates will also be considered who have a Masters degree with extensive 
experience or equivalent

We offer competitive salaries plus extensive benefits including annual bonus, 
pension plan, private medical and dental cover.

If you feel that your skills and experience match what we are looking for, 
please apply via the website: http://www.evotec.com http://www.evotec.com/


[EvotecLogoMail]
Dr. Paul A. McEwan
Principal Scientist, Structural Biology
+44. (0)1235 838802
+44. (0)1235 863139 (Fax)
paul.mce...@evotec.commailto:paul.mce...@evotec.com
www.evotec.comhttp://www.evotec.com/

Evotec (UK) Ltd.
114 Innovation Drive
Milton Park
Abingdon
Oxfordshire
OX14 4RZ  (United Kingdom)


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.

[ccp4bb] crystallography position open at Evotec

[McEwan, Paul]

Dear All, 

We have a great opportunity for somebody to join the structural biology
team at Evotec as a crystallographer. The closing date for applications
is the 31st March. Please make applications via the website as listed in
the advert below.

 

Best regards,

Paul..

 

 

 

 

Crystallographer

 

Would you like to work in exciting, cutting edge research? Why not join
the 

X-Ray Crystallographers within the Structural Biology Department at 

Evotec (UK) Ltd

 

Location: Oxfordshire, UK

Full time; Permanent

Salary: Competitive

 

Evotec (UK) Ltd is currently seeking X-Ray Crystallographers for our
Structural Biology Department based in Oxfordshire. X-Ray
Crystallography works closely with our Discovery Chemistry Department
and with clients to develop novel small molecule drugs.  The group is at
the forefront of new science and technology, and is seeking to support
its continued expansion.

 

 The successful candidates will be part of the Structural Biology group
responsible for expression, purification, crystallisation and structure
determination of proteins and protein-ligand complexes. 

 

Desirable Knowledge, Skills and Abilities:

 

* Knowledge of state of the art crystallographic methods

* Skills in molecular biology and protein purification

* Expertise in protein-ligand complex crystallisation problems
would be an advantage though not essential

* Excellent communication and interpersonal skills

* Client interaction and project management skills

 

Education and Experience:

 

* PhD qualified or equivalent in Chemistry, Biochemistry,
Molecular Biology or Biophysics

* Experience in protein X-ray crystallography

* Candidates will also be considered who have a Masters degree
with extensive experience or equivalent

 

We offer competitive salaries plus extensive benefits including annual
bonus, pension plan, private medical and dental cover.

If you feel that your skills and experience match what we are looking
for, please apply via the website: http://www.evotec.com
http://www.evotec.com 

 

 

 

Dr. Paul A. McEwan

Senior Scientist I, Structural Biology

+44. (0)1235 838802

+44. (0)1235 863139 (Fax)

paul.mce...@evotec.com mailto:paul.mce...@evotec.com 

www.evotec.com http://www.evotec.com/ 

 

Evotec (UK) Ltd.

114 Innovation Drive

Milton Park

Abingdon

Oxfordshire 

OX14 4RZ  (United Kingdom)

 

 


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.
image001.pngimage002.png

[ccp4bb] FW: [ccp4bb] disulfide engineering

[McEwan, Paul]

Rather than looking at anti-DTT it is more important to set up an
appropriate redox system. This can be a combination of reduced and
oxidised glutathione  or cysteine. If you check some of the commercial
protein refolding screens this should give you an idea about relative
concentrations.

 

Best regards,

Paul..

 


-

Dr. Paul A. McEwan

Senior Scientist (Structural Biology)

Evotec (UK) Ltd.

114 Innovation Drive | Milton Park | Abingdon | Oxfordshire | OX14 4SA

 

email: paul.mce...@evotec.com mailto:paul.mce...@evotec.com 

Tel: +44 (0)1235 861561

Fax:+44 (0)1235 863139

direct line: +44 (0)1235 838802

www.evotec.com http://www.evotec.com 

 

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jacob Keller
Sent: 28 February 2013 11:09
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] disulfide engineering

 

Along these lines, what reagents do people use to promote disuflide
bonds, i.e., the anti-DTT?

 

JPK

On Thu, Feb 28, 2013 at 2:06 AM, David Briggs drdavidcbri...@gmail.com
wrote:

You might want to try Disulfide by design

http://cptweb.cpt.wayne.edu/DbD2/

Cheers

Dave

On Feb 28, 2013 6:55 AM, Careina Edgooms careinaedgo...@yahoo.com
wrote:

Dear CCP4 members

 

I wish to engineer a disulfide bond at the dimer interface of a protein
I am working with. Does anyone know of any available software to assist
with this?

 

Best

Careina





 

-- 
***
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: j-kell...@northwestern.edu
*** 


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Milton Park, Abingdon, 
Oxfordshire, OX14 4SA, United Kingdom.

[ccp4bb] small lysozyme crystals

[McEwan Paul]

Use the 15 minute protocol from the Hampton Research catalogue/website. They're normally quite small and easy to cryoprtect with a little glycerol. Size can also be varied by playing with the salt concentration (slightly higher [NaCl]giving more but smaller xtals)

Paul..


###
Dr. Paul A. McEwan
Protein Crystallography Group
Office C58
Centre for Biomolecular Science
University of Nottingham
Nottingham
NG7 2RD
UK
Tel: 0115 8232018
http://www.nottingham.ac.uk/pharmacy/research/medicinal-chemistry-structural-biology/structbio.php###

This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
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Re: [ccp4bb] Structural importance of ordered water?

[McEwan Paul]

Title: Re: [ccp4bb] Fw: Re: [ccp4bb] Structural importance of ordered water?



Some work has been done on collagen regardingits hydration structure:
(crystal data) Bella,J.Brodsky, B. Berman, H.M.. Structure (3), 893-906 1995
(NMR) Peto, S. Gillis, P. Henri, V.P.. Biophys J. (57), 71-84, 1990
(NMR) Renou, J.P. Bonnet, M. Bielicki, G. Rochdi, A. Gatellier, P. Biopolymers (34), 1615-1626, 1994




###
Dr. Paul A. McEwan
Protein Crystallography Group
Office C58
Centre for Biomolecular Science
University of Nottingham
Nottingham
NG7 2RD
UK
Tel: 0115 8232018
http://www.nottingham.ac.uk/pharmacy/research/medicinal-chemistry-structural-biology/structbio.php###


This message has been checked for viruses but the contents of an attachment
may still contain software viruses, which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation.